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From local resynchronization to global pattern recovery in the zebrafish segmentation clock
Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the oscillating tissue complements local synchr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984840/ https://www.ncbi.nlm.nih.gov/pubmed/33587039 http://dx.doi.org/10.7554/eLife.61358 |
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author | Uriu, Koichiro Liao, Bo-Kai Oates, Andrew C Morelli, Luis G |
author_facet | Uriu, Koichiro Liao, Bo-Kai Oates, Andrew C Morelli, Luis G |
author_sort | Uriu, Koichiro |
collection | PubMed |
description | Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the oscillating tissue complements local synchronization during patterning and segment formation is not understood. We combine theory and experiment to investigate this question in the zebrafish segmentation clock. We remove a Notch inhibitor, allowing resynchronization, and analyze embryonic segment recovery. We observe unexpected intermingling of normal and defective segments, and capture this with a new model combining coupled oscillators and tissue mechanics. Intermingled segments are explained in the theory by advection of persistent phase vortices of oscillators. Experimentally observed changes in recovery patterns are predicted in the theory by temporal changes in tissue length and cell advection pattern. Thus, segmental pattern recovery occurs at two length and time scales: rapid local synchronization between neighboring cells, and the slower transport of the resulting patterns across the tissue through morphogenesis. |
format | Online Article Text |
id | pubmed-7984840 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79848402021-03-24 From local resynchronization to global pattern recovery in the zebrafish segmentation clock Uriu, Koichiro Liao, Bo-Kai Oates, Andrew C Morelli, Luis G eLife Developmental Biology Integrity of rhythmic spatial gene expression patterns in the vertebrate segmentation clock requires local synchronization between neighboring cells by Delta-Notch signaling and its inhibition causes defective segment boundaries. Whether deformation of the oscillating tissue complements local synchronization during patterning and segment formation is not understood. We combine theory and experiment to investigate this question in the zebrafish segmentation clock. We remove a Notch inhibitor, allowing resynchronization, and analyze embryonic segment recovery. We observe unexpected intermingling of normal and defective segments, and capture this with a new model combining coupled oscillators and tissue mechanics. Intermingled segments are explained in the theory by advection of persistent phase vortices of oscillators. Experimentally observed changes in recovery patterns are predicted in the theory by temporal changes in tissue length and cell advection pattern. Thus, segmental pattern recovery occurs at two length and time scales: rapid local synchronization between neighboring cells, and the slower transport of the resulting patterns across the tissue through morphogenesis. eLife Sciences Publications, Ltd 2021-02-15 /pmc/articles/PMC7984840/ /pubmed/33587039 http://dx.doi.org/10.7554/eLife.61358 Text en © 2021, Uriu et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Uriu, Koichiro Liao, Bo-Kai Oates, Andrew C Morelli, Luis G From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title | From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title_full | From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title_fullStr | From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title_full_unstemmed | From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title_short | From local resynchronization to global pattern recovery in the zebrafish segmentation clock |
title_sort | from local resynchronization to global pattern recovery in the zebrafish segmentation clock |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984840/ https://www.ncbi.nlm.nih.gov/pubmed/33587039 http://dx.doi.org/10.7554/eLife.61358 |
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