Cargando…
HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus
Huo-Xue-Jiang-Tang Yin (HXJTY) is a Chinese medicine formulation, which has been widely used for the treatment of various lipometabolism- and glycometabolism-related diseases in clinics. Currently, HXJTY is mainly prescribed to treat patients with type 2 diabetes mellitus (T2DM), yet its chemical an...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984925/ https://www.ncbi.nlm.nih.gov/pubmed/33790972 http://dx.doi.org/10.1155/2021/1072975 |
_version_ | 1783668137959358464 |
---|---|
author | Chen, Qiugu Zhao, Yuan Li, Maosheng Zheng, Ping Zhang, Shangbin Li, Huilin Chen, Jianping |
author_facet | Chen, Qiugu Zhao, Yuan Li, Maosheng Zheng, Ping Zhang, Shangbin Li, Huilin Chen, Jianping |
author_sort | Chen, Qiugu |
collection | PubMed |
description | Huo-Xue-Jiang-Tang Yin (HXJTY) is a Chinese medicine formulation, which has been widely used for the treatment of various lipometabolism- and glycometabolism-related diseases in clinics. Currently, HXJTY is mainly prescribed to treat patients with type 2 diabetes mellitus (T2DM), yet its chemical and pharmacologic profiles remain to be elucidated. Here, the potential bioactive compound and action mechanism were investigated using chemical and network pharmacology analysis. A rapid HPLC-MS was employed to identify and quantify the component of HXJTY. On the basis of the identified chemical markers from HXJTY, a network pharmacology study, including target gene prediction and functional enrichment, was applied to screen out the main quality markers of HXJTY and explore its potential mechanism for the treatment of T2DM. The results showed that a total of 22 components were identified and quantified from HXJTY by HPLC-MS. Furthermore, 12 active components such as astragaloside IV, calycosin-7-O-β-D-glucoside, hydroxysafflor yellow A, and others were proposed as quality markers of HXJTY for treating T2DM based on network pharmacology analysis. In addition, 125 corresponding possible therapeutic target genes of T2DM were obtained. These target genes are mainly related to peptidase activity, hydrolase activity, phosphatase activity, and cofactor binding, suggesting the involvement of PI3K-Akt, MAPK, AGE-RAGE, and Rap1 signaling pathways in HXJTY-treated T2DM. Our results may provide a useful approach to identify potential quality markers and molecular mechanism of HXJTY for treating T2DM. |
format | Online Article Text |
id | pubmed-7984925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-79849252021-03-30 HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus Chen, Qiugu Zhao, Yuan Li, Maosheng Zheng, Ping Zhang, Shangbin Li, Huilin Chen, Jianping Evid Based Complement Alternat Med Research Article Huo-Xue-Jiang-Tang Yin (HXJTY) is a Chinese medicine formulation, which has been widely used for the treatment of various lipometabolism- and glycometabolism-related diseases in clinics. Currently, HXJTY is mainly prescribed to treat patients with type 2 diabetes mellitus (T2DM), yet its chemical and pharmacologic profiles remain to be elucidated. Here, the potential bioactive compound and action mechanism were investigated using chemical and network pharmacology analysis. A rapid HPLC-MS was employed to identify and quantify the component of HXJTY. On the basis of the identified chemical markers from HXJTY, a network pharmacology study, including target gene prediction and functional enrichment, was applied to screen out the main quality markers of HXJTY and explore its potential mechanism for the treatment of T2DM. The results showed that a total of 22 components were identified and quantified from HXJTY by HPLC-MS. Furthermore, 12 active components such as astragaloside IV, calycosin-7-O-β-D-glucoside, hydroxysafflor yellow A, and others were proposed as quality markers of HXJTY for treating T2DM based on network pharmacology analysis. In addition, 125 corresponding possible therapeutic target genes of T2DM were obtained. These target genes are mainly related to peptidase activity, hydrolase activity, phosphatase activity, and cofactor binding, suggesting the involvement of PI3K-Akt, MAPK, AGE-RAGE, and Rap1 signaling pathways in HXJTY-treated T2DM. Our results may provide a useful approach to identify potential quality markers and molecular mechanism of HXJTY for treating T2DM. Hindawi 2021-03-15 /pmc/articles/PMC7984925/ /pubmed/33790972 http://dx.doi.org/10.1155/2021/1072975 Text en Copyright © 2021 Qiugu Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chen, Qiugu Zhao, Yuan Li, Maosheng Zheng, Ping Zhang, Shangbin Li, Huilin Chen, Jianping HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title | HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title_full | HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title_fullStr | HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title_full_unstemmed | HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title_short | HPLC-MS and Network Pharmacology Analysis to Reveal Quality Markers of Huo-Xue-Jiang-Tang Yin, a Chinese Herbal Medicine for Type 2 Diabetes Mellitus |
title_sort | hplc-ms and network pharmacology analysis to reveal quality markers of huo-xue-jiang-tang yin, a chinese herbal medicine for type 2 diabetes mellitus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984925/ https://www.ncbi.nlm.nih.gov/pubmed/33790972 http://dx.doi.org/10.1155/2021/1072975 |
work_keys_str_mv | AT chenqiugu hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT zhaoyuan hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT limaosheng hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT zhengping hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT zhangshangbin hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT lihuilin hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus AT chenjianping hplcmsandnetworkpharmacologyanalysistorevealqualitymarkersofhuoxuejiangtangyinachineseherbalmedicinefortype2diabetesmellitus |