Cargando…

Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons

Plasma rich in growth factors (PRGF) is a subtype of platelet-rich plasma (PRP) that stimulates tissue regeneration and may promote neuronal survival. It has been employed in ophthalmology to achieve tissue repair in some retinal pathologies, although how PRGF acts in the retina is still poorly unde...

Descripción completa

Detalles Bibliográficos
Autores principales: Ruzafa, Noelia, Pereiro, Xandra, Fonollosa, Alex, Araiz, Javier, Acera, Arantxa, Vecino, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985077/
https://www.ncbi.nlm.nih.gov/pubmed/33767620
http://dx.doi.org/10.3389/fphar.2021.606275
_version_ 1783668165354455040
author Ruzafa, Noelia
Pereiro, Xandra
Fonollosa, Alex
Araiz, Javier
Acera, Arantxa
Vecino, Elena
author_facet Ruzafa, Noelia
Pereiro, Xandra
Fonollosa, Alex
Araiz, Javier
Acera, Arantxa
Vecino, Elena
author_sort Ruzafa, Noelia
collection PubMed
description Plasma rich in growth factors (PRGF) is a subtype of platelet-rich plasma (PRP) that stimulates tissue regeneration and may promote neuronal survival. It has been employed in ophthalmology to achieve tissue repair in some retinal pathologies, although how PRGF acts in the retina is still poorly understood. As a part of the central nervous system, the retina has limited capacity for repair capacity following damage, and retinal insult can provoke the death of retinal ganglion cells (RGCs), potentially producing irreversible blindness. RGCs are in close contact with glial cells, such as Müller cells, that help maintain homeostasis in the retina. In this study, the aim was to determine whether PRGF can protect RGCs and whether it increases the number of Müller cells. Therefore, PRGF were tested on primary cell cultures of porcine RGCs and Müller cells, as well as on co-cultures of these two cell types. Moreover, the inflammatory component of PRGF was analyzed and the cytokines in the different PRGFs were quantified. In addition, we set out to determine if blocking the inflammatory components of PRGF alters its effect on the cells in culture. The presence of PRGF compromises RGC survival in pure cultures and in co-culture with Müller cells, but this effect was reversed by heat-inactivation of the PRGF. The detrimental effect of PRGF on RGCs could be in part due to the presence of cytokines and specifically, to the presence of pro-inflammatory cytokines that compromise their survival. However, other factors are likely to be present in the PRGF that have a deleterious effect on the RGCs since the exposure to antibodies against these cytokines were insufficient to protect RGCs. Moreover, PRGF promotes Müller cell survival. In conclusion, PRGF hinders the survival of RGCs in the presence or absence of Müller cells, yet it promotes Müller cell survival that could be the reason of retina healing observed in the in vivo treatments, with some cytokines possibly implicated. Although PRGF could stimulate tissue regeneration, further studies should be performed to evaluate the effect of PRGF on neurons and the implication of its potential inflammatory role in such processes.
format Online
Article
Text
id pubmed-7985077
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79850772021-03-24 Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons Ruzafa, Noelia Pereiro, Xandra Fonollosa, Alex Araiz, Javier Acera, Arantxa Vecino, Elena Front Pharmacol Pharmacology Plasma rich in growth factors (PRGF) is a subtype of platelet-rich plasma (PRP) that stimulates tissue regeneration and may promote neuronal survival. It has been employed in ophthalmology to achieve tissue repair in some retinal pathologies, although how PRGF acts in the retina is still poorly understood. As a part of the central nervous system, the retina has limited capacity for repair capacity following damage, and retinal insult can provoke the death of retinal ganglion cells (RGCs), potentially producing irreversible blindness. RGCs are in close contact with glial cells, such as Müller cells, that help maintain homeostasis in the retina. In this study, the aim was to determine whether PRGF can protect RGCs and whether it increases the number of Müller cells. Therefore, PRGF were tested on primary cell cultures of porcine RGCs and Müller cells, as well as on co-cultures of these two cell types. Moreover, the inflammatory component of PRGF was analyzed and the cytokines in the different PRGFs were quantified. In addition, we set out to determine if blocking the inflammatory components of PRGF alters its effect on the cells in culture. The presence of PRGF compromises RGC survival in pure cultures and in co-culture with Müller cells, but this effect was reversed by heat-inactivation of the PRGF. The detrimental effect of PRGF on RGCs could be in part due to the presence of cytokines and specifically, to the presence of pro-inflammatory cytokines that compromise their survival. However, other factors are likely to be present in the PRGF that have a deleterious effect on the RGCs since the exposure to antibodies against these cytokines were insufficient to protect RGCs. Moreover, PRGF promotes Müller cell survival. In conclusion, PRGF hinders the survival of RGCs in the presence or absence of Müller cells, yet it promotes Müller cell survival that could be the reason of retina healing observed in the in vivo treatments, with some cytokines possibly implicated. Although PRGF could stimulate tissue regeneration, further studies should be performed to evaluate the effect of PRGF on neurons and the implication of its potential inflammatory role in such processes. Frontiers Media S.A. 2021-03-09 /pmc/articles/PMC7985077/ /pubmed/33767620 http://dx.doi.org/10.3389/fphar.2021.606275 Text en Copyright © 2021 Ruzafa, Pereiro, Fonollosa, Araiz, Acera and Vecino. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ruzafa, Noelia
Pereiro, Xandra
Fonollosa, Alex
Araiz, Javier
Acera, Arantxa
Vecino, Elena
Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title_full Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title_fullStr Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title_full_unstemmed Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title_short Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons
title_sort plasma rich in growth factors (prgf) increases the number of retinal müller glia in culture but not the survival of retinal neurons
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985077/
https://www.ncbi.nlm.nih.gov/pubmed/33767620
http://dx.doi.org/10.3389/fphar.2021.606275
work_keys_str_mv AT ruzafanoelia plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons
AT pereiroxandra plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons
AT fonollosaalex plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons
AT araizjavier plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons
AT aceraarantxa plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons
AT vecinoelena plasmarichingrowthfactorsprgfincreasesthenumberofretinalmullergliainculturebutnotthesurvivalofretinalneurons