Cargando…

Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease

Rheumatic heart disease refers to the long-term damage of heart valves and results from an autoimmune response to group A Streptococcus infection. This study aimed to analyze the microbiota composition of patients with rheumatic heart disease and explore potential function of microbiota in this dise...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Xue-Rui, Chen, Bo-Yan, Lin, Wen-Zhen, Li, Yu-Lin, Wang, Yong-Li, Liu, Yan, Huang, Jing-Juan, Zhang, Wei-Wei, Ma, Xiao-Xin, Shao, Shuai, Li, Ruo-Gu, Duan, Sheng-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985333/
https://www.ncbi.nlm.nih.gov/pubmed/33768014
http://dx.doi.org/10.3389/fcimb.2021.643092
_version_ 1783668223934201856
author Shi, Xue-Rui
Chen, Bo-Yan
Lin, Wen-Zhen
Li, Yu-Lin
Wang, Yong-Li
Liu, Yan
Huang, Jing-Juan
Zhang, Wei-Wei
Ma, Xiao-Xin
Shao, Shuai
Li, Ruo-Gu
Duan, Sheng-Zhong
author_facet Shi, Xue-Rui
Chen, Bo-Yan
Lin, Wen-Zhen
Li, Yu-Lin
Wang, Yong-Li
Liu, Yan
Huang, Jing-Juan
Zhang, Wei-Wei
Ma, Xiao-Xin
Shao, Shuai
Li, Ruo-Gu
Duan, Sheng-Zhong
author_sort Shi, Xue-Rui
collection PubMed
description Rheumatic heart disease refers to the long-term damage of heart valves and results from an autoimmune response to group A Streptococcus infection. This study aimed to analyze the microbiota composition of patients with rheumatic heart disease and explore potential function of microbiota in this disease. First, we revealed significant alterations of microbiota in feces, subgingival plaques, and saliva of the patients compared to control subjects using 16S rRNA gene sequencing. Significantly different microbial diversity was observed in all three types of samples between the patients and control subjects. In the gut, the patients possessed higher levels of genera including Bifidobacterium and Eubacterium, and lower levels of genera including Lachnospira, Bacteroides, and Faecalibacterium. Coprococcus was identified as a super-generalist in fecal samples of the patients. Significant alterations were also observed in microbiota of subgingival plaques and saliva of the patients compared to control subjects. Second, we analyzed microbiota in mitral valves of the patients and identified microbes that could potentially transmit from the gut or oral cavity to heart valves, including Streptococcus. Third, we further analyzed the data using random forest model and demonstrated that microbiota in the gut, subgingival plaque or saliva could distinguish the patients from control subjects. Finally, we identified gut/oral microbes that significantly correlated with clinical indices of rheumatic heart disease. In conclusion, patients with rheumatic heart disease manifested important alterations in microbiota that might distinguish the patients from control subjects and correlated with severity of this disease.
format Online
Article
Text
id pubmed-7985333
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79853332021-03-24 Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease Shi, Xue-Rui Chen, Bo-Yan Lin, Wen-Zhen Li, Yu-Lin Wang, Yong-Li Liu, Yan Huang, Jing-Juan Zhang, Wei-Wei Ma, Xiao-Xin Shao, Shuai Li, Ruo-Gu Duan, Sheng-Zhong Front Cell Infect Microbiol Cellular and Infection Microbiology Rheumatic heart disease refers to the long-term damage of heart valves and results from an autoimmune response to group A Streptococcus infection. This study aimed to analyze the microbiota composition of patients with rheumatic heart disease and explore potential function of microbiota in this disease. First, we revealed significant alterations of microbiota in feces, subgingival plaques, and saliva of the patients compared to control subjects using 16S rRNA gene sequencing. Significantly different microbial diversity was observed in all three types of samples between the patients and control subjects. In the gut, the patients possessed higher levels of genera including Bifidobacterium and Eubacterium, and lower levels of genera including Lachnospira, Bacteroides, and Faecalibacterium. Coprococcus was identified as a super-generalist in fecal samples of the patients. Significant alterations were also observed in microbiota of subgingival plaques and saliva of the patients compared to control subjects. Second, we analyzed microbiota in mitral valves of the patients and identified microbes that could potentially transmit from the gut or oral cavity to heart valves, including Streptococcus. Third, we further analyzed the data using random forest model and demonstrated that microbiota in the gut, subgingival plaque or saliva could distinguish the patients from control subjects. Finally, we identified gut/oral microbes that significantly correlated with clinical indices of rheumatic heart disease. In conclusion, patients with rheumatic heart disease manifested important alterations in microbiota that might distinguish the patients from control subjects and correlated with severity of this disease. Frontiers Media S.A. 2021-03-09 /pmc/articles/PMC7985333/ /pubmed/33768014 http://dx.doi.org/10.3389/fcimb.2021.643092 Text en Copyright © 2021 Shi, Chen, Lin, Li, Wang, Liu, Huang, Zhang, Ma, Shao, Li and Duan http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Shi, Xue-Rui
Chen, Bo-Yan
Lin, Wen-Zhen
Li, Yu-Lin
Wang, Yong-Li
Liu, Yan
Huang, Jing-Juan
Zhang, Wei-Wei
Ma, Xiao-Xin
Shao, Shuai
Li, Ruo-Gu
Duan, Sheng-Zhong
Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title_full Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title_fullStr Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title_full_unstemmed Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title_short Microbiota in Gut, Oral Cavity, and Mitral Valves Are Associated With Rheumatic Heart Disease
title_sort microbiota in gut, oral cavity, and mitral valves are associated with rheumatic heart disease
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985333/
https://www.ncbi.nlm.nih.gov/pubmed/33768014
http://dx.doi.org/10.3389/fcimb.2021.643092
work_keys_str_mv AT shixuerui microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT chenboyan microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT linwenzhen microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT liyulin microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT wangyongli microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT liuyan microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT huangjingjuan microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT zhangweiwei microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT maxiaoxin microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT shaoshuai microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT liruogu microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease
AT duanshengzhong microbiotaingutoralcavityandmitralvalvesareassociatedwithrheumaticheartdisease