Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans

Live-cell imaging analysis provides tremendous information for the study of cellular events such as growth cone migration in neuronal development. Here, we describe a protocol for live-cell imaging of migrating PVD dendritic growth cones in the nematode C. elegans by spinning-disk confocal microscop...

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Autores principales: Chen, Chun-Hao, Pan, Chun-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985398/
https://www.ncbi.nlm.nih.gov/pubmed/33778786
http://dx.doi.org/10.1016/j.xpro.2021.100402
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author Chen, Chun-Hao
Pan, Chun-Liang
author_facet Chen, Chun-Hao
Pan, Chun-Liang
author_sort Chen, Chun-Hao
collection PubMed
description Live-cell imaging analysis provides tremendous information for the study of cellular events such as growth cone migration in neuronal development. Here, we describe a protocol for live-cell imaging of migrating PVD dendritic growth cones in the nematode C. elegans by spinning-disk confocal microscopy. Fluorescently labeled growth cones and cytoskeletal proteins could be continuously observed for 4–6 h in mid-stage larvae. This protocol is suitable for revealing the dynamic molecular and cellular events in dendrite and axon development of C. elegans. For complete details on the use and execution of this protocol, please refer to Chen et al. (2019).
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spelling pubmed-79853982021-03-25 Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans Chen, Chun-Hao Pan, Chun-Liang STAR Protoc Protocol Live-cell imaging analysis provides tremendous information for the study of cellular events such as growth cone migration in neuronal development. Here, we describe a protocol for live-cell imaging of migrating PVD dendritic growth cones in the nematode C. elegans by spinning-disk confocal microscopy. Fluorescently labeled growth cones and cytoskeletal proteins could be continuously observed for 4–6 h in mid-stage larvae. This protocol is suitable for revealing the dynamic molecular and cellular events in dendrite and axon development of C. elegans. For complete details on the use and execution of this protocol, please refer to Chen et al. (2019). Elsevier 2021-03-18 /pmc/articles/PMC7985398/ /pubmed/33778786 http://dx.doi.org/10.1016/j.xpro.2021.100402 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Chun-Hao
Pan, Chun-Liang
Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title_full Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title_fullStr Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title_full_unstemmed Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title_short Live-cell imaging of PVD dendritic growth cone in post-embryonic C. elegans
title_sort live-cell imaging of pvd dendritic growth cone in post-embryonic c. elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985398/
https://www.ncbi.nlm.nih.gov/pubmed/33778786
http://dx.doi.org/10.1016/j.xpro.2021.100402
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AT panchunliang livecellimagingofpvddendriticgrowthconeinpostembryoniccelegans

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