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Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women

Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoki...

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Autores principales: Piaggeschi, Giulia, Rolla, Simona, Rossi, Niccolò, Brusa, Davide, Naccarati, Alessio, Couvreur, Simon, Spector, Tim D., Roederer, Mario, Mangino, Massimo, Cordero, Francesca, Falchi, Mario, Visconti, Alessia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985448/
https://www.ncbi.nlm.nih.gov/pubmed/33767708
http://dx.doi.org/10.3389/fimmu.2021.637974
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author Piaggeschi, Giulia
Rolla, Simona
Rossi, Niccolò
Brusa, Davide
Naccarati, Alessio
Couvreur, Simon
Spector, Tim D.
Roederer, Mario
Mangino, Massimo
Cordero, Francesca
Falchi, Mario
Visconti, Alessia
author_facet Piaggeschi, Giulia
Rolla, Simona
Rossi, Niccolò
Brusa, Davide
Naccarati, Alessio
Couvreur, Simon
Spector, Tim D.
Roederer, Mario
Mangino, Massimo
Cordero, Francesca
Falchi, Mario
Visconti, Alessia
author_sort Piaggeschi, Giulia
collection PubMed
description Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.
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spelling pubmed-79854482021-03-24 Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women Piaggeschi, Giulia Rolla, Simona Rossi, Niccolò Brusa, Davide Naccarati, Alessio Couvreur, Simon Spector, Tim D. Roederer, Mario Mangino, Massimo Cordero, Francesca Falchi, Mario Visconti, Alessia Front Immunol Immunology Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation. Frontiers Media S.A. 2021-03-09 /pmc/articles/PMC7985448/ /pubmed/33767708 http://dx.doi.org/10.3389/fimmu.2021.637974 Text en Copyright © 2021 Piaggeschi, Rolla, Rossi, Brusa, Naccarati, Couvreur, Spector, Roederer, Mangino, Cordero, Falchi and Visconti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Piaggeschi, Giulia
Rolla, Simona
Rossi, Niccolò
Brusa, Davide
Naccarati, Alessio
Couvreur, Simon
Spector, Tim D.
Roederer, Mario
Mangino, Massimo
Cordero, Francesca
Falchi, Mario
Visconti, Alessia
Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title_full Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title_fullStr Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title_full_unstemmed Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title_short Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women
title_sort immune trait shifts in association with tobacco smoking: a study in healthy women
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985448/
https://www.ncbi.nlm.nih.gov/pubmed/33767708
http://dx.doi.org/10.3389/fimmu.2021.637974
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