Alginate-based 3D cancer cell culture for therapeutic response modeling

Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) c...

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Detalles Bibliográficos
Autores principales: Davoudi, Farideh, Ghorbanpoor, Samar, Yoda, Satoshi, Pan, Xiao, Crowther, Giovanna Stein, Yin, Xunqin, Murchie, Ellen, Hata, Aaron N., Willers, Henning, Benes, Cyril H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985559/
https://www.ncbi.nlm.nih.gov/pubmed/33778784
http://dx.doi.org/10.1016/j.xpro.2021.100391
Descripción
Sumario:Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017), Lehmann et al. (2016), Liu et al. (2016), and Duval et al. (2011).

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