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Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response

Plants regulate their reproductive cycles under the influence of environmental cues, such as day length, temperature and water availability. In Solanum tuberosum (potato), vegetative reproduction via tuberization is known to be regulated by photoperiod, in a very similar way to flowering. The centra...

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Autores principales: Ramírez Gonzales, Lorena, Shi, Li, Bergonzi, Sara Bergonzi, Oortwijn, Marian, Franco‐Zorrilla, José M., Solano‐Tavira, Roberto, Visser, Richard G. F., Abelenda, José A., Bachem, Christian W. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985872/
https://www.ncbi.nlm.nih.gov/pubmed/33220113
http://dx.doi.org/10.1111/tpj.15093
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author Ramírez Gonzales, Lorena
Shi, Li
Bergonzi, Sara Bergonzi
Oortwijn, Marian
Franco‐Zorrilla, José M.
Solano‐Tavira, Roberto
Visser, Richard G. F.
Abelenda, José A.
Bachem, Christian W. B.
author_facet Ramírez Gonzales, Lorena
Shi, Li
Bergonzi, Sara Bergonzi
Oortwijn, Marian
Franco‐Zorrilla, José M.
Solano‐Tavira, Roberto
Visser, Richard G. F.
Abelenda, José A.
Bachem, Christian W. B.
author_sort Ramírez Gonzales, Lorena
collection PubMed
description Plants regulate their reproductive cycles under the influence of environmental cues, such as day length, temperature and water availability. In Solanum tuberosum (potato), vegetative reproduction via tuberization is known to be regulated by photoperiod, in a very similar way to flowering. The central clock output transcription factor CYCLING DOF FACTOR 1 (StCDF1) was shown to regulate tuberization. We now show that StCDF1, together with a long non‐coding RNA (lncRNA) counterpart, named StFLORE, also regulates water loss through affecting stomatal growth and diurnal opening. Both natural and CRISPR‐Cas9 mutations in the StFLORE transcript produce plants with increased sensitivity to water‐limiting conditions. Conversely, elevated expression of StFLORE, both by the overexpression of StFLORE or by the downregulation of StCDF1, results in an increased tolerance to drought through reducing water loss. Although StFLORE appears to act as a natural antisense transcript, it is in turn regulated by the StCDF1 transcription factor. We further show that StCDF1 is a non‐redundant regulator of tuberization that affects the expression of two other members of the potato StCDF gene family, as well as StCO genes, through binding to a canonical sequence motif. Taken together, we demonstrate that the StCDF1–StFLORE locus is important for vegetative reproduction and water homeostasis, both of which are important traits for potato plant breeding.
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spelling pubmed-79858722021-03-25 Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response Ramírez Gonzales, Lorena Shi, Li Bergonzi, Sara Bergonzi Oortwijn, Marian Franco‐Zorrilla, José M. Solano‐Tavira, Roberto Visser, Richard G. F. Abelenda, José A. Bachem, Christian W. B. Plant J Original Article Plants regulate their reproductive cycles under the influence of environmental cues, such as day length, temperature and water availability. In Solanum tuberosum (potato), vegetative reproduction via tuberization is known to be regulated by photoperiod, in a very similar way to flowering. The central clock output transcription factor CYCLING DOF FACTOR 1 (StCDF1) was shown to regulate tuberization. We now show that StCDF1, together with a long non‐coding RNA (lncRNA) counterpart, named StFLORE, also regulates water loss through affecting stomatal growth and diurnal opening. Both natural and CRISPR‐Cas9 mutations in the StFLORE transcript produce plants with increased sensitivity to water‐limiting conditions. Conversely, elevated expression of StFLORE, both by the overexpression of StFLORE or by the downregulation of StCDF1, results in an increased tolerance to drought through reducing water loss. Although StFLORE appears to act as a natural antisense transcript, it is in turn regulated by the StCDF1 transcription factor. We further show that StCDF1 is a non‐redundant regulator of tuberization that affects the expression of two other members of the potato StCDF gene family, as well as StCO genes, through binding to a canonical sequence motif. Taken together, we demonstrate that the StCDF1–StFLORE locus is important for vegetative reproduction and water homeostasis, both of which are important traits for potato plant breeding. John Wiley and Sons Inc. 2021-02-11 2021-02 /pmc/articles/PMC7985872/ /pubmed/33220113 http://dx.doi.org/10.1111/tpj.15093 Text en © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Article
Ramírez Gonzales, Lorena
Shi, Li
Bergonzi, Sara Bergonzi
Oortwijn, Marian
Franco‐Zorrilla, José M.
Solano‐Tavira, Roberto
Visser, Richard G. F.
Abelenda, José A.
Bachem, Christian W. B.
Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title_full Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title_fullStr Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title_full_unstemmed Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title_short Potato CYCLING DOF FACTOR 1 and its lncRNA counterpart StFLORE link tuber development and drought response
title_sort potato cycling dof factor 1 and its lncrna counterpart stflore link tuber development and drought response
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985872/
https://www.ncbi.nlm.nih.gov/pubmed/33220113
http://dx.doi.org/10.1111/tpj.15093
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