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Long-term stabilization of DNA at room temperature using a one-step microwave assisted process
Long-term stabilization of DNA is needed for forensic, clinical, in-field operations and numerous other applications. Although freezing (<−20 °C) and dry storage are currently the preferential methods for long-term storage, a noticeable pre-analytical degradation of DNA over time, upfront capital...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986179/ https://www.ncbi.nlm.nih.gov/pubmed/33778372 http://dx.doi.org/10.1007/s42247-021-00208-3 |
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author | Narvaez Villarrubia, Claudia W. Tumas, Keyla C. Chauhan, Rajat MacDonald, Thomas Dattelbaum, Andrew M. Omberg, Kristin Gupta, Gautam |
author_facet | Narvaez Villarrubia, Claudia W. Tumas, Keyla C. Chauhan, Rajat MacDonald, Thomas Dattelbaum, Andrew M. Omberg, Kristin Gupta, Gautam |
author_sort | Narvaez Villarrubia, Claudia W. |
collection | PubMed |
description | Long-term stabilization of DNA is needed for forensic, clinical, in-field operations and numerous other applications. Although freezing (<−20 °C) and dry storage are currently the preferential methods for long-term storage, a noticeable pre-analytical degradation of DNA over time, upfront capital investment and recurring costs have demonstrated a need for an alternative long-term room-temperature preservation method. Herein, we report a novel, fast (~5 min) silica sol–gel preparation method using a standard microwave-initiated polymerization reaction amenable to stabilization of DNA. The method involves use of one chemical, tetramethoxy silane (TMOS) and eliminates the use of alcohol as co-solvent and catalysts such as acids. In addition, the process involves minimal technical expertise, thus making it an ideal choice for resource-challenged countries and in-field applications. The sol–gel is capable to store and stabilize Escherichia coli DNA in ambient conditions for 210 days. DNA recovered from the sol–gel showed no significant nucleolytic and/or oxidative degradation, outperforming conventional storage conditions at −20 °C, and reported state-of-the-art technology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42247-021-00208-3. |
format | Online Article Text |
id | pubmed-7986179 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-79861792021-03-24 Long-term stabilization of DNA at room temperature using a one-step microwave assisted process Narvaez Villarrubia, Claudia W. Tumas, Keyla C. Chauhan, Rajat MacDonald, Thomas Dattelbaum, Andrew M. Omberg, Kristin Gupta, Gautam Emergent Mater Original Article Long-term stabilization of DNA is needed for forensic, clinical, in-field operations and numerous other applications. Although freezing (<−20 °C) and dry storage are currently the preferential methods for long-term storage, a noticeable pre-analytical degradation of DNA over time, upfront capital investment and recurring costs have demonstrated a need for an alternative long-term room-temperature preservation method. Herein, we report a novel, fast (~5 min) silica sol–gel preparation method using a standard microwave-initiated polymerization reaction amenable to stabilization of DNA. The method involves use of one chemical, tetramethoxy silane (TMOS) and eliminates the use of alcohol as co-solvent and catalysts such as acids. In addition, the process involves minimal technical expertise, thus making it an ideal choice for resource-challenged countries and in-field applications. The sol–gel is capable to store and stabilize Escherichia coli DNA in ambient conditions for 210 days. DNA recovered from the sol–gel showed no significant nucleolytic and/or oxidative degradation, outperforming conventional storage conditions at −20 °C, and reported state-of-the-art technology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42247-021-00208-3. Springer International Publishing 2021-03-23 2022 /pmc/articles/PMC7986179/ /pubmed/33778372 http://dx.doi.org/10.1007/s42247-021-00208-3 Text en © Qatar University and Springer Nature Switzerland AG 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Narvaez Villarrubia, Claudia W. Tumas, Keyla C. Chauhan, Rajat MacDonald, Thomas Dattelbaum, Andrew M. Omberg, Kristin Gupta, Gautam Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title | Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title_full | Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title_fullStr | Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title_full_unstemmed | Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title_short | Long-term stabilization of DNA at room temperature using a one-step microwave assisted process |
title_sort | long-term stabilization of dna at room temperature using a one-step microwave assisted process |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986179/ https://www.ncbi.nlm.nih.gov/pubmed/33778372 http://dx.doi.org/10.1007/s42247-021-00208-3 |
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