Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L(−1)) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation m...

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Autores principales: Gao, Fengzheng, Sá, Marta, Teles (Cabanelas, ITD), Iago, Wijffels, René H., Barbosa, Maria J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986402/
https://www.ncbi.nlm.nih.gov/pubmed/33325031
http://dx.doi.org/10.1002/bit.27657
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author Gao, Fengzheng
Sá, Marta
Teles (Cabanelas, ITD), Iago
Wijffels, René H.
Barbosa, Maria J.
author_facet Gao, Fengzheng
Sá, Marta
Teles (Cabanelas, ITD), Iago
Wijffels, René H.
Barbosa, Maria J.
author_sort Gao, Fengzheng
collection PubMed
description The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L(−1)) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R (2) for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L(−1): 75.66 and 98.14 mg L(−1) d(−1), for T. lutea and P. tricornutum, respectively; 0.4 g L(−1): 129.9 and 158.47 mg L(−1) d(−1), T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L(−1): 1.14 and 1.41 mg L(−1) d(−1), T. lutea and P. tricornutum; 0.4 g L(−1): 2.09 and 1.73 mg L(−1) d(−1), T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.
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spelling pubmed-79864022021-03-25 Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions Gao, Fengzheng Sá, Marta Teles (Cabanelas, ITD), Iago Wijffels, René H. Barbosa, Maria J. Biotechnol Bioeng ARTICLES The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L(−1)) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R (2) for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L(−1): 75.66 and 98.14 mg L(−1) d(−1), for T. lutea and P. tricornutum, respectively; 0.4 g L(−1): 129.9 and 158.47 mg L(−1) d(−1), T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L(−1): 1.14 and 1.41 mg L(−1) d(−1), T. lutea and P. tricornutum; 0.4 g L(−1): 2.09 and 1.73 mg L(−1) d(−1), T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization. John Wiley and Sons Inc. 2020-12-31 2021-03 /pmc/articles/PMC7986402/ /pubmed/33325031 http://dx.doi.org/10.1002/bit.27657 Text en © 2020 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle ARTICLES
Gao, Fengzheng
Sá, Marta
Teles (Cabanelas, ITD), Iago
Wijffels, René H.
Barbosa, Maria J.
Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title_full Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title_fullStr Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title_full_unstemmed Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title_short Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
title_sort production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
topic ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986402/
https://www.ncbi.nlm.nih.gov/pubmed/33325031
http://dx.doi.org/10.1002/bit.27657
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