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Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles
The MinDE proteins from E. coli have received great attention as a paradigmatic biological pattern‐forming system. Recently, it has surfaced that these proteins do not only generate oscillating concentration gradients driven by ATP hydrolysis, but that they can reversibly deform giant vesicles. In o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986748/ https://www.ncbi.nlm.nih.gov/pubmed/33285025 http://dx.doi.org/10.1002/anie.202015184 |
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author | Fu, Meifang Franquelim, Henri G. Kretschmer, Simon Schwille, Petra |
author_facet | Fu, Meifang Franquelim, Henri G. Kretschmer, Simon Schwille, Petra |
author_sort | Fu, Meifang |
collection | PubMed |
description | The MinDE proteins from E. coli have received great attention as a paradigmatic biological pattern‐forming system. Recently, it has surfaced that these proteins do not only generate oscillating concentration gradients driven by ATP hydrolysis, but that they can reversibly deform giant vesicles. In order to explore the potential of Min proteins to actually perform mechanical work, we introduce a new model membrane system, flat vesicle stacks on top of a supported lipid bilayer. MinDE oscillations can repeatedly deform these flat vesicles into tubules and promote progressive membrane spreading through membrane adhesion. Dependent on membrane and buffer compositions, Min oscillations further induce robust bud formation. Altogether, we demonstrate that under specific conditions, MinDE self‐organization can result in work performed on biomimetic systems and achieve a straightforward mechanochemical coupling between the MinDE biochemical reaction cycle and membrane transformation. |
format | Online Article Text |
id | pubmed-7986748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79867482021-03-25 Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles Fu, Meifang Franquelim, Henri G. Kretschmer, Simon Schwille, Petra Angew Chem Int Ed Engl Research Articles The MinDE proteins from E. coli have received great attention as a paradigmatic biological pattern‐forming system. Recently, it has surfaced that these proteins do not only generate oscillating concentration gradients driven by ATP hydrolysis, but that they can reversibly deform giant vesicles. In order to explore the potential of Min proteins to actually perform mechanical work, we introduce a new model membrane system, flat vesicle stacks on top of a supported lipid bilayer. MinDE oscillations can repeatedly deform these flat vesicles into tubules and promote progressive membrane spreading through membrane adhesion. Dependent on membrane and buffer compositions, Min oscillations further induce robust bud formation. Altogether, we demonstrate that under specific conditions, MinDE self‐organization can result in work performed on biomimetic systems and achieve a straightforward mechanochemical coupling between the MinDE biochemical reaction cycle and membrane transformation. John Wiley and Sons Inc. 2021-01-26 2021-03-15 /pmc/articles/PMC7986748/ /pubmed/33285025 http://dx.doi.org/10.1002/anie.202015184 Text en © 2020 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Fu, Meifang Franquelim, Henri G. Kretschmer, Simon Schwille, Petra Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title | Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title_full | Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title_fullStr | Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title_full_unstemmed | Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title_short | Non‐Equilibrium Large‐Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles |
title_sort | non‐equilibrium large‐scale membrane transformations driven by minde biochemical reaction cycles |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986748/ https://www.ncbi.nlm.nih.gov/pubmed/33285025 http://dx.doi.org/10.1002/anie.202015184 |
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