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Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC Pig‐a Workgroup
The rodent Pig‐a assay is a flow cytometric, phenotype‐based method used to measure in vivo somatic cell mutation. An Organization for Economic Co‐operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project nu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986863/ https://www.ncbi.nlm.nih.gov/pubmed/33608913 http://dx.doi.org/10.1002/em.22427 |
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author | Dertinger, Stephen D. Bhalli, Javed A. Roberts, Daniel J. Stankowski, Leon F. Gollapudi, B. Bhaskar Lovell, David P. Recio, Leslie Kimoto, Takafumi Miura, Daishiro Heflich, Robert H. |
author_facet | Dertinger, Stephen D. Bhalli, Javed A. Roberts, Daniel J. Stankowski, Leon F. Gollapudi, B. Bhaskar Lovell, David P. Recio, Leslie Kimoto, Takafumi Miura, Daishiro Heflich, Robert H. |
author_sort | Dertinger, Stephen D. |
collection | PubMed |
description | The rodent Pig‐a assay is a flow cytometric, phenotype‐based method used to measure in vivo somatic cell mutation. An Organization for Economic Co‐operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number 4.93). This article provides advice on best practices for designing and conducting rodent Pig‐a studies in support of evaluating test substance safety, with a focus on the rat model. Various aspects of assay conduct, including laboratory proficiency, minimum number of animals per dose group, preferred treatment and blood sampling schedule, and statistical analysis are described. |
format | Online Article Text |
id | pubmed-7986863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79868632021-03-25 Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC Pig‐a Workgroup Dertinger, Stephen D. Bhalli, Javed A. Roberts, Daniel J. Stankowski, Leon F. Gollapudi, B. Bhaskar Lovell, David P. Recio, Leslie Kimoto, Takafumi Miura, Daishiro Heflich, Robert H. Environ Mol Mutagen Review The rodent Pig‐a assay is a flow cytometric, phenotype‐based method used to measure in vivo somatic cell mutation. An Organization for Economic Co‐operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number 4.93). This article provides advice on best practices for designing and conducting rodent Pig‐a studies in support of evaluating test substance safety, with a focus on the rat model. Various aspects of assay conduct, including laboratory proficiency, minimum number of animals per dose group, preferred treatment and blood sampling schedule, and statistical analysis are described. John Wiley & Sons, Inc. 2021-03-02 2021-03 /pmc/articles/PMC7986863/ /pubmed/33608913 http://dx.doi.org/10.1002/em.22427 Text en © 2021 The Authors. Environmental and Molecular Mutagenesis published by Wiley Periodicals LLC on behalf of Environmental Mutagen Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Dertinger, Stephen D. Bhalli, Javed A. Roberts, Daniel J. Stankowski, Leon F. Gollapudi, B. Bhaskar Lovell, David P. Recio, Leslie Kimoto, Takafumi Miura, Daishiro Heflich, Robert H. Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC Pig‐a Workgroup |
title | Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC
Pig‐a Workgroup |
title_full | Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC
Pig‐a Workgroup |
title_fullStr | Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC
Pig‐a Workgroup |
title_full_unstemmed | Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC
Pig‐a Workgroup |
title_short | Recommendations for conducting the rodent erythrocyte Pig‐a assay: A report from the HESI GTTC
Pig‐a Workgroup |
title_sort | recommendations for conducting the rodent erythrocyte pig‐a assay: a report from the hesi gttc
pig‐a workgroup |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986863/ https://www.ncbi.nlm.nih.gov/pubmed/33608913 http://dx.doi.org/10.1002/em.22427 |
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