A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection

Emerging SARS-CoV-2 variants have raised concerns about resistance to neutralizing antibodies elicited by previous infection or vaccination. We examined whether sera from recovered and naive donors collected prior to, and following immunizations with existing mRNA vaccines, could neutralize the Wuha...

Descripción completa

Detalles Bibliográficos
Autores principales: Stamatatos, Leonidas, Czartoski, Julie, Wan, Yu-Hsin, Homad, Leah J., Rubin, Vanessa, Glantz, Hayley, Neradilek, Moni, Seydoux, Emilie, Jennewein, Madeleine F., MacCamy, Anna J., Feng, Junli, Mize, Gregory, De Rosa, Stephen C., Finzi, Andrés, Lemos, Maria P., Cohen, Kristen W., Moodie, Zoe, McElrath, M. Juliana, McGuire, Andrew T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987032/
https://www.ncbi.nlm.nih.gov/pubmed/33758873
http://dx.doi.org/10.1101/2021.02.05.21251182
_version_ 1783668551870054400
author Stamatatos, Leonidas
Czartoski, Julie
Wan, Yu-Hsin
Homad, Leah J.
Rubin, Vanessa
Glantz, Hayley
Neradilek, Moni
Seydoux, Emilie
Jennewein, Madeleine F.
MacCamy, Anna J.
Feng, Junli
Mize, Gregory
De Rosa, Stephen C.
Finzi, Andrés
Lemos, Maria P.
Cohen, Kristen W.
Moodie, Zoe
McElrath, M. Juliana
McGuire, Andrew T.
author_facet Stamatatos, Leonidas
Czartoski, Julie
Wan, Yu-Hsin
Homad, Leah J.
Rubin, Vanessa
Glantz, Hayley
Neradilek, Moni
Seydoux, Emilie
Jennewein, Madeleine F.
MacCamy, Anna J.
Feng, Junli
Mize, Gregory
De Rosa, Stephen C.
Finzi, Andrés
Lemos, Maria P.
Cohen, Kristen W.
Moodie, Zoe
McElrath, M. Juliana
McGuire, Andrew T.
author_sort Stamatatos, Leonidas
collection PubMed
description Emerging SARS-CoV-2 variants have raised concerns about resistance to neutralizing antibodies elicited by previous infection or vaccination. We examined whether sera from recovered and naive donors collected prior to, and following immunizations with existing mRNA vaccines, could neutralize the Wuhan-Hu-1 and B.1.351 variants. Pre-vaccination sera from recovered donors neutralized Wuhan-Hu-1 and sporadically neutralized B.1.351, but a single immunization boosted neutralizing titers against all variants and SARS-CoV-1 by up to 1000-fold. Neutralization was due to antibodies targeting the receptor binding domain and was not boosted by a second immunization. Immunization of naïve donors also elicited cross-neutralizing responses, but at lower titers. Our study highlights the importance of vaccinating both uninfected and previously infected persons to elicit cross-variant neutralizing antibodies.
format Online
Article
Text
id pubmed-7987032
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-79870322021-03-24 A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection Stamatatos, Leonidas Czartoski, Julie Wan, Yu-Hsin Homad, Leah J. Rubin, Vanessa Glantz, Hayley Neradilek, Moni Seydoux, Emilie Jennewein, Madeleine F. MacCamy, Anna J. Feng, Junli Mize, Gregory De Rosa, Stephen C. Finzi, Andrés Lemos, Maria P. Cohen, Kristen W. Moodie, Zoe McElrath, M. Juliana McGuire, Andrew T. medRxiv Article Emerging SARS-CoV-2 variants have raised concerns about resistance to neutralizing antibodies elicited by previous infection or vaccination. We examined whether sera from recovered and naive donors collected prior to, and following immunizations with existing mRNA vaccines, could neutralize the Wuhan-Hu-1 and B.1.351 variants. Pre-vaccination sera from recovered donors neutralized Wuhan-Hu-1 and sporadically neutralized B.1.351, but a single immunization boosted neutralizing titers against all variants and SARS-CoV-1 by up to 1000-fold. Neutralization was due to antibodies targeting the receptor binding domain and was not boosted by a second immunization. Immunization of naïve donors also elicited cross-neutralizing responses, but at lower titers. Our study highlights the importance of vaccinating both uninfected and previously infected persons to elicit cross-variant neutralizing antibodies. Cold Spring Harbor Laboratory 2021-03-10 /pmc/articles/PMC7987032/ /pubmed/33758873 http://dx.doi.org/10.1101/2021.02.05.21251182 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Stamatatos, Leonidas
Czartoski, Julie
Wan, Yu-Hsin
Homad, Leah J.
Rubin, Vanessa
Glantz, Hayley
Neradilek, Moni
Seydoux, Emilie
Jennewein, Madeleine F.
MacCamy, Anna J.
Feng, Junli
Mize, Gregory
De Rosa, Stephen C.
Finzi, Andrés
Lemos, Maria P.
Cohen, Kristen W.
Moodie, Zoe
McElrath, M. Juliana
McGuire, Andrew T.
A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title_full A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title_fullStr A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title_full_unstemmed A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title_short A single mRNA immunization boosts cross-variant neutralizing antibodies elicited by SARS-CoV-2 infection
title_sort single mrna immunization boosts cross-variant neutralizing antibodies elicited by sars-cov-2 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987032/
https://www.ncbi.nlm.nih.gov/pubmed/33758873
http://dx.doi.org/10.1101/2021.02.05.21251182
work_keys_str_mv AT stamatatosleonidas asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT czartoskijulie asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT wanyuhsin asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT homadleahj asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT rubinvanessa asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT glantzhayley asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT neradilekmoni asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT seydouxemilie asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT jenneweinmadeleinef asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT maccamyannaj asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT fengjunli asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mizegregory asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT derosastephenc asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT finziandres asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT lemosmariap asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT cohenkristenw asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT moodiezoe asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mcelrathmjuliana asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mcguireandrewt asinglemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT stamatatosleonidas singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT czartoskijulie singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT wanyuhsin singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT homadleahj singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT rubinvanessa singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT glantzhayley singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT neradilekmoni singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT seydouxemilie singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT jenneweinmadeleinef singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT maccamyannaj singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT fengjunli singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mizegregory singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT derosastephenc singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT finziandres singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT lemosmariap singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT cohenkristenw singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT moodiezoe singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mcelrathmjuliana singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection
AT mcguireandrewt singlemrnaimmunizationboostscrossvariantneutralizingantibodieselicitedbysarscov2infection

Ejemplares similares