LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion

PURPOSE: Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. METHODS: The expression of RBM5-AS1 i...

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Autores principales: Deng, Biyong, Pan, Runsang, Ou, Xin, Wang, Taizhe, Wang, Weiguo, Nie, Yingjie, Chen, Houping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987434/
https://www.ncbi.nlm.nih.gov/pubmed/33816613
http://dx.doi.org/10.1155/2021/5271291
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author Deng, Biyong
Pan, Runsang
Ou, Xin
Wang, Taizhe
Wang, Weiguo
Nie, Yingjie
Chen, Houping
author_facet Deng, Biyong
Pan, Runsang
Ou, Xin
Wang, Taizhe
Wang, Weiguo
Nie, Yingjie
Chen, Houping
author_sort Deng, Biyong
collection PubMed
description PURPOSE: Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. METHODS: The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5. RESULTS: LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells. CONCLUSION: To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os.
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spelling pubmed-79874342021-04-02 LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion Deng, Biyong Pan, Runsang Ou, Xin Wang, Taizhe Wang, Weiguo Nie, Yingjie Chen, Houping Biomed Res Int Research Article PURPOSE: Osteosarcoma (Os) is the most frequent malignant tumor of the bone in the pediatric age group, and accumulating evidences show that lncRNAs play a key role in the development of Os. Thus, we investigated the role of RBM5-AS1 and its molecular mechanism. METHODS: The expression of RBM5-AS1 in Os tissues and cell lines was detected by real-time polymerase chain reaction (QPCR). The effect of RBM5-AS1 on the proliferation of Os cells was detected using CCK8 assays and flow cytometry. The effect of RBM5-AS1 on the migration and invasion of Os cells was detected by transwell assays. And we performed QPCR and western blotting assays to investigate the relationship between RBM5-AS1 and RBM5. Finally, western blotting assays were performed to explore the mechanism of RBM5. RESULTS: LncRNA RBM5-AS1 was overexpressed in the Os tissues and cell lines. And lncRNA RBM5-AS1 promoted Os cell proliferation, migration, and invasion in vitro and tumor growth in vivo. LncRNA RBM5-AS1 targets RBM5 in Os cells. CONCLUSION: To sum up, the results showed that lncRNA RBM5-AS1 promotes cell proliferation, migration, and invasion in Os. Hindawi 2021-03-15 /pmc/articles/PMC7987434/ /pubmed/33816613 http://dx.doi.org/10.1155/2021/5271291 Text en Copyright © 2021 Biyong Deng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Deng, Biyong
Pan, Runsang
Ou, Xin
Wang, Taizhe
Wang, Weiguo
Nie, Yingjie
Chen, Houping
LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title_full LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title_fullStr LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title_full_unstemmed LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title_short LncRNA RBM5-AS1 Promotes Osteosarcoma Cell Proliferation, Migration, and Invasion
title_sort lncrna rbm5-as1 promotes osteosarcoma cell proliferation, migration, and invasion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987434/
https://www.ncbi.nlm.nih.gov/pubmed/33816613
http://dx.doi.org/10.1155/2021/5271291
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