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Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function
Objective: The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration. Method: Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone lo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987667/ https://www.ncbi.nlm.nih.gov/pubmed/33776769 http://dx.doi.org/10.3389/fphar.2021.626621 |
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author | Zhao, Lu Du, Wei Zhao, Dandan Ji, Xueyan Huang, Yanfei Pang, Yong Guo, Kaijin Yin, Xiaoxing |
author_facet | Zhao, Lu Du, Wei Zhao, Dandan Ji, Xueyan Huang, Yanfei Pang, Yong Guo, Kaijin Yin, Xiaoxing |
author_sort | Zhao, Lu |
collection | PubMed |
description | Objective: The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration. Method: Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone loss, architectural deterioration of trabecular bone and bone metabolism biomarkers were tested. A model of MC3T3-E1 cells was established by treatment with high glucose; the regulatory role of catalpol in the differentiation and migration was tested by Western blot, ALP staining, and Alizarin Red staining. Results: Catalpol treatment markedly ameliorated trabecular bone deterioration by reducing degenerative changes of the trabecular structure by improving the bone formation marker levels of ALP, osteopontin, type I collagen, and osteocalcin, as well as the level of OPG/RANKL. Catalpol enhanced cell motility and scattering following gap formation of MC3T3-E1 cells. Conclusion: The results indicated that catalpol exhibits a protective effect against diabetic osteoporosis by regulating the differentiation and migration of osteoblast. |
format | Online Article Text |
id | pubmed-7987667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79876672021-03-25 Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function Zhao, Lu Du, Wei Zhao, Dandan Ji, Xueyan Huang, Yanfei Pang, Yong Guo, Kaijin Yin, Xiaoxing Front Pharmacol Pharmacology Objective: The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration. Method: Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone loss, architectural deterioration of trabecular bone and bone metabolism biomarkers were tested. A model of MC3T3-E1 cells was established by treatment with high glucose; the regulatory role of catalpol in the differentiation and migration was tested by Western blot, ALP staining, and Alizarin Red staining. Results: Catalpol treatment markedly ameliorated trabecular bone deterioration by reducing degenerative changes of the trabecular structure by improving the bone formation marker levels of ALP, osteopontin, type I collagen, and osteocalcin, as well as the level of OPG/RANKL. Catalpol enhanced cell motility and scattering following gap formation of MC3T3-E1 cells. Conclusion: The results indicated that catalpol exhibits a protective effect against diabetic osteoporosis by regulating the differentiation and migration of osteoblast. Frontiers Media S.A. 2021-03-10 /pmc/articles/PMC7987667/ /pubmed/33776769 http://dx.doi.org/10.3389/fphar.2021.626621 Text en Copyright © 2021 Zhao, Du, Zhao, Ji, Huang, Pang, Guo and Yin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Zhao, Lu Du, Wei Zhao, Dandan Ji, Xueyan Huang, Yanfei Pang, Yong Guo, Kaijin Yin, Xiaoxing Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title | Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title_full | Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title_fullStr | Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title_full_unstemmed | Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title_short | Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function |
title_sort | catalpol protects against high glucose-induced bone loss by regulating osteoblast function |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987667/ https://www.ncbi.nlm.nih.gov/pubmed/33776769 http://dx.doi.org/10.3389/fphar.2021.626621 |
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