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Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite

Herein, in a one-pot method, the reduced graphene oxide layers with the assistance of multiwalled carbon nanotubes were decorated to provide a suitable space for the in situ growth of CoNi(2)S(4), and the porphyrins were incorporated into the layers as well to increase the sensitivity of the prepare...

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Autores principales: Rabiee, Navid, Bagherzadeh, Mojtaba, Ghadiri, Amir Mohammad, Fatahi, Yousef, Baheiraei, Nafiseh, Safarkhani, Moein, Aldhaher, Abdullah, Dinarvand, Rassoul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988124/
https://www.ncbi.nlm.nih.gov/pubmed/33758300
http://dx.doi.org/10.1038/s41598-021-86119-z
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author Rabiee, Navid
Bagherzadeh, Mojtaba
Ghadiri, Amir Mohammad
Fatahi, Yousef
Baheiraei, Nafiseh
Safarkhani, Moein
Aldhaher, Abdullah
Dinarvand, Rassoul
author_facet Rabiee, Navid
Bagherzadeh, Mojtaba
Ghadiri, Amir Mohammad
Fatahi, Yousef
Baheiraei, Nafiseh
Safarkhani, Moein
Aldhaher, Abdullah
Dinarvand, Rassoul
author_sort Rabiee, Navid
collection PubMed
description Herein, in a one-pot method, the reduced graphene oxide layers with the assistance of multiwalled carbon nanotubes were decorated to provide a suitable space for the in situ growth of CoNi(2)S(4), and the porphyrins were incorporated into the layers as well to increase the sensitivity of the prepared nanostructure. The prepared nanocomposite can establish π–π interactions between the genetic material and on the surface of porphyrin rings. Also, hydrogen bonds between genetic domains and the porphyrin’ nitrogen and the surface hydroxyl groups are probable. Furthermore, the potential donor–acceptor relationship between the d(7) transition metal, cobalt, and the genetic material provides a suitable way to increase the interaction and gene loading , and transfections. The reason for this phenomenon was optimized to increase the EGFP by up to 17.9%. Furthermore, the sensing ability of the nanocomposite towards H(2)O(2) was investigated. In this regard, the limit of detection of the H(2)O(2) obtained 10 µM. Also, the in situ biosensing ability in the HEK-293 and PC12 cell lines was evaluated by the addition of PMA. The nanocomposite showed the ability to detect the released H(2)O(2) after adding the minimum amount of 120 ng/mL of the PMA.
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spelling pubmed-79881242021-03-25 Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite Rabiee, Navid Bagherzadeh, Mojtaba Ghadiri, Amir Mohammad Fatahi, Yousef Baheiraei, Nafiseh Safarkhani, Moein Aldhaher, Abdullah Dinarvand, Rassoul Sci Rep Article Herein, in a one-pot method, the reduced graphene oxide layers with the assistance of multiwalled carbon nanotubes were decorated to provide a suitable space for the in situ growth of CoNi(2)S(4), and the porphyrins were incorporated into the layers as well to increase the sensitivity of the prepared nanostructure. The prepared nanocomposite can establish π–π interactions between the genetic material and on the surface of porphyrin rings. Also, hydrogen bonds between genetic domains and the porphyrin’ nitrogen and the surface hydroxyl groups are probable. Furthermore, the potential donor–acceptor relationship between the d(7) transition metal, cobalt, and the genetic material provides a suitable way to increase the interaction and gene loading , and transfections. The reason for this phenomenon was optimized to increase the EGFP by up to 17.9%. Furthermore, the sensing ability of the nanocomposite towards H(2)O(2) was investigated. In this regard, the limit of detection of the H(2)O(2) obtained 10 µM. Also, the in situ biosensing ability in the HEK-293 and PC12 cell lines was evaluated by the addition of PMA. The nanocomposite showed the ability to detect the released H(2)O(2) after adding the minimum amount of 120 ng/mL of the PMA. Nature Publishing Group UK 2021-03-23 /pmc/articles/PMC7988124/ /pubmed/33758300 http://dx.doi.org/10.1038/s41598-021-86119-z Text en © The Author(s) 2021, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rabiee, Navid
Bagherzadeh, Mojtaba
Ghadiri, Amir Mohammad
Fatahi, Yousef
Baheiraei, Nafiseh
Safarkhani, Moein
Aldhaher, Abdullah
Dinarvand, Rassoul
Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title_full Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title_fullStr Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title_full_unstemmed Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title_short Bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
title_sort bio-multifunctional noncovalent porphyrin functionalized carbon-based nanocomposite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988124/
https://www.ncbi.nlm.nih.gov/pubmed/33758300
http://dx.doi.org/10.1038/s41598-021-86119-z
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