Cargando…
Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP
We used palaeoproteomics and peptide mass fingerprinting to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. It can be difficult to distinguish fragmentary remains of early domesticates (sheep) from similar-sized local wild...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988125/ https://www.ncbi.nlm.nih.gov/pubmed/33758223 http://dx.doi.org/10.1038/s41598-021-85756-8 |
_version_ | 1783668729483100160 |
---|---|
author | Coutu, Ashley N. Taurozzi, Alberto J. Mackie, Meaghan Jensen, Theis Zetner Trolle Collins, Matthew J. Sealy, Judith |
author_facet | Coutu, Ashley N. Taurozzi, Alberto J. Mackie, Meaghan Jensen, Theis Zetner Trolle Collins, Matthew J. Sealy, Judith |
author_sort | Coutu, Ashley N. |
collection | PubMed |
description | We used palaeoproteomics and peptide mass fingerprinting to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. It can be difficult to distinguish fragmentary remains of early domesticates (sheep) from similar-sized local wild bovids (grey duiker, grey rhebok, springbok—southern Africa lacks wild sheep) based on morphology alone. Our analysis revealed a Zooarchaeology by Mass Spectrometry (ZooMS) marker (m/z 1532) present in wild bovids and we demonstrate through LC–MS/MS that it is capable of discriminating between wild bovids and caprine domesticates. We confirm that the Spoegrivier specimen dated to 2105 ± 65 BP is indeed a sheep. This is the earliest directly dated evidence of domesticated animals in southern Africa. As well as the traditional method of analysing bone fragments, we show the utility of minimally destructive sampling methods such as PVC eraser and polishing films for successful ZooMS identification. We also show that collagen extracted more than 25 years ago for the purpose of radiocarbon dating can yield successful ZooMS identification. Our study demonstrates the importance of developing appropriate regional frameworks of comparison for future research using ZooMS as a method of biomolecular species identification. |
format | Online Article Text |
id | pubmed-7988125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79881252021-03-25 Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP Coutu, Ashley N. Taurozzi, Alberto J. Mackie, Meaghan Jensen, Theis Zetner Trolle Collins, Matthew J. Sealy, Judith Sci Rep Article We used palaeoproteomics and peptide mass fingerprinting to obtain secure species identifications of key specimens of early domesticated fauna from South Africa, dating to ca. 2000 BP. It can be difficult to distinguish fragmentary remains of early domesticates (sheep) from similar-sized local wild bovids (grey duiker, grey rhebok, springbok—southern Africa lacks wild sheep) based on morphology alone. Our analysis revealed a Zooarchaeology by Mass Spectrometry (ZooMS) marker (m/z 1532) present in wild bovids and we demonstrate through LC–MS/MS that it is capable of discriminating between wild bovids and caprine domesticates. We confirm that the Spoegrivier specimen dated to 2105 ± 65 BP is indeed a sheep. This is the earliest directly dated evidence of domesticated animals in southern Africa. As well as the traditional method of analysing bone fragments, we show the utility of minimally destructive sampling methods such as PVC eraser and polishing films for successful ZooMS identification. We also show that collagen extracted more than 25 years ago for the purpose of radiocarbon dating can yield successful ZooMS identification. Our study demonstrates the importance of developing appropriate regional frameworks of comparison for future research using ZooMS as a method of biomolecular species identification. Nature Publishing Group UK 2021-03-23 /pmc/articles/PMC7988125/ /pubmed/33758223 http://dx.doi.org/10.1038/s41598-021-85756-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Coutu, Ashley N. Taurozzi, Alberto J. Mackie, Meaghan Jensen, Theis Zetner Trolle Collins, Matthew J. Sealy, Judith Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title | Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title_full | Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title_fullStr | Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title_full_unstemmed | Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title_short | Palaeoproteomics confirm earliest domesticated sheep in southern Africa ca. 2000 BP |
title_sort | palaeoproteomics confirm earliest domesticated sheep in southern africa ca. 2000 bp |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988125/ https://www.ncbi.nlm.nih.gov/pubmed/33758223 http://dx.doi.org/10.1038/s41598-021-85756-8 |
work_keys_str_mv | AT coutuashleyn palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp AT taurozzialbertoj palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp AT mackiemeaghan palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp AT jensentheiszetnertrolle palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp AT collinsmatthewj palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp AT sealyjudith palaeoproteomicsconfirmearliestdomesticatedsheepinsouthernafricaca2000bp |