An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple...

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Detalles Bibliográficos
Autores principales: Lyra-Leite, Davi Marco, Fonoudi, Hananeh, Gharib, Mennat, Burridge, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988236/
https://www.ncbi.nlm.nih.gov/pubmed/33786455
http://dx.doi.org/10.1016/j.xpro.2020.100213
Descripción
Sumario:The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).

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