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An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells
The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple...
Autores principales: | Lyra-Leite, Davi Marco, Fonoudi, Hananeh, Gharib, Mennat, Burridge, Paul W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988236/ https://www.ncbi.nlm.nih.gov/pubmed/33786455 http://dx.doi.org/10.1016/j.xpro.2020.100213 |
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