Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as b...

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Detalles Bibliográficos
Autores principales: Bojmar, Linda, Kim, Han Sang, Tobias, Gabriel C., Pelissier Vatter, Fanny A., Lucotti, Serena, Gyan, Kofi Ennu, Kenific, Candia M., Wan, Zurong, Kim, Kyung-A, Kim, DooA, Hernandez, Jonathan, Pascual, Virginia, Heaton, Todd E., La Quaglia, Michael P., Kelsen, David, Trippett, Tanya M., Jones, David R., Jarnagin, William R., Matei, Irina R., Zhang, Haiying, Hoshino, Ayuko, Lyden, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988237/
https://www.ncbi.nlm.nih.gov/pubmed/33786456
http://dx.doi.org/10.1016/j.xpro.2020.100225
Descripción
Sumario:We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).

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