A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome

This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which co...

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Detalles Bibliográficos
Autores principales: Payling, Laura, Roy, Nicole C., Fraser, Karl, Loveday, Simon M., Sims, Ian M., Janssen, Peter H., Hill, Stefan J., Raymond, Laura G., McNabb, Warren C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988238/
https://www.ncbi.nlm.nih.gov/pubmed/33786457
http://dx.doi.org/10.1016/j.xpro.2020.100227
Descripción
Sumario:This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.

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