A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome

This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which co...

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Autores principales: Payling, Laura, Roy, Nicole C., Fraser, Karl, Loveday, Simon M., Sims, Ian M., Janssen, Peter H., Hill, Stefan J., Raymond, Laura G., McNabb, Warren C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988238/
https://www.ncbi.nlm.nih.gov/pubmed/33786457
http://dx.doi.org/10.1016/j.xpro.2020.100227
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author Payling, Laura
Roy, Nicole C.
Fraser, Karl
Loveday, Simon M.
Sims, Ian M.
Janssen, Peter H.
Hill, Stefan J.
Raymond, Laura G.
McNabb, Warren C.
author_facet Payling, Laura
Roy, Nicole C.
Fraser, Karl
Loveday, Simon M.
Sims, Ian M.
Janssen, Peter H.
Hill, Stefan J.
Raymond, Laura G.
McNabb, Warren C.
author_sort Payling, Laura
collection PubMed
description This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.
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spelling pubmed-79882382021-03-29 A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome Payling, Laura Roy, Nicole C. Fraser, Karl Loveday, Simon M. Sims, Ian M. Janssen, Peter H. Hill, Stefan J. Raymond, Laura G. McNabb, Warren C. STAR Protoc Protocol This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions. Elsevier 2020-12-24 /pmc/articles/PMC7988238/ /pubmed/33786457 http://dx.doi.org/10.1016/j.xpro.2020.100227 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Payling, Laura
Roy, Nicole C.
Fraser, Karl
Loveday, Simon M.
Sims, Ian M.
Janssen, Peter H.
Hill, Stefan J.
Raymond, Laura G.
McNabb, Warren C.
A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title_full A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title_fullStr A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title_full_unstemmed A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title_short A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
title_sort protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988238/
https://www.ncbi.nlm.nih.gov/pubmed/33786457
http://dx.doi.org/10.1016/j.xpro.2020.100227
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