CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcript...

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Detalles Bibliográficos
Autores principales: Hoppe, Caroline, Ashe, Hilary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988240/
https://www.ncbi.nlm.nih.gov/pubmed/33786461
http://dx.doi.org/10.1016/j.xpro.2021.100380
Descripción
Sumario:CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila. For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).

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