CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcript...

Descripción completa

Detalles Bibliográficos
Autores principales: Hoppe, Caroline, Ashe, Hilary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988240/
https://www.ncbi.nlm.nih.gov/pubmed/33786461
http://dx.doi.org/10.1016/j.xpro.2021.100380
_version_ 1783668755463667712
author Hoppe, Caroline
Ashe, Hilary L.
author_facet Hoppe, Caroline
Ashe, Hilary L.
author_sort Hoppe, Caroline
collection PubMed
description CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila. For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020).
format Online
Article
Text
id pubmed-7988240
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-79882402021-03-29 CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos Hoppe, Caroline Ashe, Hilary L. STAR Protoc Protocol CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in Drosophila. This can facilitate live imaging of nascent transcription in Drosophila. For complete details on the use and execution of this protocol, please refer to Hoppe et al. (2020). Elsevier 2021-03-18 /pmc/articles/PMC7988240/ /pubmed/33786461 http://dx.doi.org/10.1016/j.xpro.2021.100380 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Hoppe, Caroline
Ashe, Hilary L.
CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_full CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_fullStr CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_full_unstemmed CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_short CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in Drosophila embryos
title_sort crispr-cas9 strategies to insert ms2 stem-loops into endogenous loci in drosophila embryos
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988240/
https://www.ncbi.nlm.nih.gov/pubmed/33786461
http://dx.doi.org/10.1016/j.xpro.2021.100380
work_keys_str_mv AT hoppecaroline crisprcas9strategiestoinsertms2stemloopsintoendogenouslociindrosophilaembryos
AT ashehilaryl crisprcas9strategiestoinsertms2stemloopsintoendogenouslociindrosophilaembryos

Ejemplares similares