Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia

We developed a next-generation SARS-CoV-2 sequencing platform and obtained the first SARS-CoV-2 sequences from patients in Croatia at the beginning of the COVID-19 outbreak in the spring of 2020. Integrating the sequencing and the epidemiological data, we show that patients were infected with differ...

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Autores principales: Rokić, Filip, Trgovec-Greif, Lovro, Sučić, Neven, Čemeljić, Noa, Grbeša, Đurđica Cekinović, Svedružić, Željko, Rukavina, Tomislav, Vugrek, Oliver, Jurak, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988246/
https://www.ncbi.nlm.nih.gov/pubmed/33761008
http://dx.doi.org/10.1007/s00705-021-05029-7
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author Rokić, Filip
Trgovec-Greif, Lovro
Sučić, Neven
Čemeljić, Noa
Grbeša, Đurđica Cekinović
Svedružić, Željko
Rukavina, Tomislav
Vugrek, Oliver
Jurak, Igor
author_facet Rokić, Filip
Trgovec-Greif, Lovro
Sučić, Neven
Čemeljić, Noa
Grbeša, Đurđica Cekinović
Svedružić, Željko
Rukavina, Tomislav
Vugrek, Oliver
Jurak, Igor
author_sort Rokić, Filip
collection PubMed
description We developed a next-generation SARS-CoV-2 sequencing platform and obtained the first SARS-CoV-2 sequences from patients in Croatia at the beginning of the COVID-19 outbreak in the spring of 2020. Integrating the sequencing and the epidemiological data, we show that patients were infected with different SARS-CoV-2 variants belonging to different clades (mostly G and GH). This result confirms that there was widespread virus transmission early in 2020. Interestingly, we identified a unique mutation resulting in a V13I substitution in Nsp5A, the main viral protease, in a patient who had not received antiviral therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05029-7.
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spelling pubmed-79882462021-03-24 Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia Rokić, Filip Trgovec-Greif, Lovro Sučić, Neven Čemeljić, Noa Grbeša, Đurđica Cekinović Svedružić, Željko Rukavina, Tomislav Vugrek, Oliver Jurak, Igor Arch Virol Brief Report We developed a next-generation SARS-CoV-2 sequencing platform and obtained the first SARS-CoV-2 sequences from patients in Croatia at the beginning of the COVID-19 outbreak in the spring of 2020. Integrating the sequencing and the epidemiological data, we show that patients were infected with different SARS-CoV-2 variants belonging to different clades (mostly G and GH). This result confirms that there was widespread virus transmission early in 2020. Interestingly, we identified a unique mutation resulting in a V13I substitution in Nsp5A, the main viral protease, in a patient who had not received antiviral therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05029-7. Springer Vienna 2021-03-24 2021 /pmc/articles/PMC7988246/ /pubmed/33761008 http://dx.doi.org/10.1007/s00705-021-05029-7 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Rokić, Filip
Trgovec-Greif, Lovro
Sučić, Neven
Čemeljić, Noa
Grbeša, Đurđica Cekinović
Svedružić, Željko
Rukavina, Tomislav
Vugrek, Oliver
Jurak, Igor
Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title_full Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title_fullStr Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title_full_unstemmed Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title_short Diverse SARS-CoV-2 variants preceded the initial COVID-19 outbreak in Croatia
title_sort diverse sars-cov-2 variants preceded the initial covid-19 outbreak in croatia
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988246/
https://www.ncbi.nlm.nih.gov/pubmed/33761008
http://dx.doi.org/10.1007/s00705-021-05029-7
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