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The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin)
BACKGROUND: In this study, we assessed the in vitro antischistosomal activity of the active ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) on Schistosoma mansoni. METHODS: This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were expos...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988669/ https://www.ncbi.nlm.nih.gov/pubmed/33786052 http://dx.doi.org/10.18502/ijpa.v16i1.5540 |
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author | RASHED, Hadeer Abd El-Hak ABU ALMAATY, Ali Hussein SOLIMAN, Maha Farid Mohamed EL-SHENAWY, Nahla Soliman |
author_facet | RASHED, Hadeer Abd El-Hak ABU ALMAATY, Ali Hussein SOLIMAN, Maha Farid Mohamed EL-SHENAWY, Nahla Soliman |
author_sort | RASHED, Hadeer Abd El-Hak |
collection | PubMed |
description | BACKGROUND: In this study, we assessed the in vitro antischistosomal activity of the active ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) on Schistosoma mansoni. METHODS: This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on S. mansoni were determined by DNA fragmentation analysis. RESULTS: We determined the concentrations of AL and CU required to kill 50% of S. mansoni (LC(50 )). The LC(50) of AL was 8.66 μL/mL, whereas 100% mortality of S. mansoni was achieved by AL at concentrations of 50 μL/mL. The LC(50) of CU was 87.25 μL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 μg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to S. mansion, respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in S. mansoni worms, we observed no genotoxic effects of AL. CONCLUSION: Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel. |
format | Online Article Text |
id | pubmed-7988669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-79886692021-03-29 The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) RASHED, Hadeer Abd El-Hak ABU ALMAATY, Ali Hussein SOLIMAN, Maha Farid Mohamed EL-SHENAWY, Nahla Soliman Iran J Parasitol Original Article BACKGROUND: In this study, we assessed the in vitro antischistosomal activity of the active ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) on Schistosoma mansoni. METHODS: This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on S. mansoni were determined by DNA fragmentation analysis. RESULTS: We determined the concentrations of AL and CU required to kill 50% of S. mansoni (LC(50 )). The LC(50) of AL was 8.66 μL/mL, whereas 100% mortality of S. mansoni was achieved by AL at concentrations of 50 μL/mL. The LC(50) of CU was 87.25 μL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 μg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to S. mansion, respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in S. mansoni worms, we observed no genotoxic effects of AL. CONCLUSION: Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel. Tehran University of Medical Sciences 2021 /pmc/articles/PMC7988669/ /pubmed/33786052 http://dx.doi.org/10.18502/ijpa.v16i1.5540 Text en Copyright © 2021 Rashed et al. Published by Tehran University of Medical Sciences https://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited. |
spellingShingle | Original Article RASHED, Hadeer Abd El-Hak ABU ALMAATY, Ali Hussein SOLIMAN, Maha Farid Mohamed EL-SHENAWY, Nahla Soliman The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title | The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title_full | The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title_fullStr | The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title_full_unstemmed | The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title_short | The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) |
title_sort | in vitro antischistosomal activity and genotoxicity of the active ingredients of allium sativum (allicin) and curcuma longa (curcumin) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988669/ https://www.ncbi.nlm.nih.gov/pubmed/33786052 http://dx.doi.org/10.18502/ijpa.v16i1.5540 |
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