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Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics

BACKGROUND: Jiawei Shengjiang Powder (JWSJP) is a classical Chinese medicinal formula, which has been widely applied in the treatment of asthma and complications for many years due to its curative effect. AIM: To verify the effect of JWSJP in improving abnormal sperm motility caused by asthma and to...

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Autores principales: Wang, Ji-sheng, Gong, Xue-feng, Feng, Jun-long, Li, Hai-song, Li, Xiao, Deng, Sheng, Ren, Pei-zhong, Wang, Jia-mei, Lv, Ming-sheng, Jin, Rui-feng, Chen, Qiu-yi, Wang, Bin, Cui, Hong-sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989703/
https://www.ncbi.nlm.nih.gov/pubmed/33776422
http://dx.doi.org/10.2147/DDDT.S296901
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author Wang, Ji-sheng
Gong, Xue-feng
Feng, Jun-long
Li, Hai-song
Li, Xiao
Deng, Sheng
Ren, Pei-zhong
Wang, Jia-mei
Lv, Ming-sheng
Jin, Rui-feng
Chen, Qiu-yi
Wang, Bin
Cui, Hong-sheng
author_facet Wang, Ji-sheng
Gong, Xue-feng
Feng, Jun-long
Li, Hai-song
Li, Xiao
Deng, Sheng
Ren, Pei-zhong
Wang, Jia-mei
Lv, Ming-sheng
Jin, Rui-feng
Chen, Qiu-yi
Wang, Bin
Cui, Hong-sheng
author_sort Wang, Ji-sheng
collection PubMed
description BACKGROUND: Jiawei Shengjiang Powder (JWSJP) is a classical Chinese medicinal formula, which has been widely applied in the treatment of asthma and complications for many years due to its curative effect. AIM: To verify the effect of JWSJP in improving abnormal sperm motility caused by asthma and to explore its potential mechanism. MATERIALS AND METHODS: The active compounds of JWSJP were obtained from high performance liquid chromatography tandem mass spectrometry and the Traditional Chinese Medicine System Pharmacology. The key active components and targets of JWSJP were predicted based on network pharmacological analysis and bioinformatics research. Rats were randomly divided into normal, model and treatment groups. The rat model of allergic asthma was induced by intraperitoneal injection of ovalbumin solution. The experiment judged improvement of semen quality by evaluating sperm motility, and detected the expression of related proteins in testicular tissue of Sprague-Dawley rats by RT-qPCR and Western blot methods. Hematoxylin and eosin (HE) staining was used to observe the changes in testicular tissue structure in rats. RESULTS: Through the analysis of network pharmacology and bioinformatics, it was found that beta-sitosterol, quercetin, gallic acid, pelargonidin and kaempferol were the key active components of Jiawei Shengjiang Powder. Tumor necrosis factor (TNF), interleukin-6 (IL-6) and insulin (INS) genes are crucial targets of JWSJP in the treatment of spermatogenic dysfunction caused by acute asthma. After 8 weeks of intervention, compared with the model group, the treatment group had significantly improved sperm motility (P < 0.05). There were significant differences in TNF, IL6, and INS proteins in the treatment group, and the HE staining of testicular tissue structure in the treatment group was significantly improved. CONCLUSION: JWSJP can improve the abnormal sperm motility induced by asthma, and its mechanism may be related to the expression of related proteins and mRNA of TNF, IL6, and INS.
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spelling pubmed-79897032021-03-25 Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics Wang, Ji-sheng Gong, Xue-feng Feng, Jun-long Li, Hai-song Li, Xiao Deng, Sheng Ren, Pei-zhong Wang, Jia-mei Lv, Ming-sheng Jin, Rui-feng Chen, Qiu-yi Wang, Bin Cui, Hong-sheng Drug Des Devel Ther Original Research BACKGROUND: Jiawei Shengjiang Powder (JWSJP) is a classical Chinese medicinal formula, which has been widely applied in the treatment of asthma and complications for many years due to its curative effect. AIM: To verify the effect of JWSJP in improving abnormal sperm motility caused by asthma and to explore its potential mechanism. MATERIALS AND METHODS: The active compounds of JWSJP were obtained from high performance liquid chromatography tandem mass spectrometry and the Traditional Chinese Medicine System Pharmacology. The key active components and targets of JWSJP were predicted based on network pharmacological analysis and bioinformatics research. Rats were randomly divided into normal, model and treatment groups. The rat model of allergic asthma was induced by intraperitoneal injection of ovalbumin solution. The experiment judged improvement of semen quality by evaluating sperm motility, and detected the expression of related proteins in testicular tissue of Sprague-Dawley rats by RT-qPCR and Western blot methods. Hematoxylin and eosin (HE) staining was used to observe the changes in testicular tissue structure in rats. RESULTS: Through the analysis of network pharmacology and bioinformatics, it was found that beta-sitosterol, quercetin, gallic acid, pelargonidin and kaempferol were the key active components of Jiawei Shengjiang Powder. Tumor necrosis factor (TNF), interleukin-6 (IL-6) and insulin (INS) genes are crucial targets of JWSJP in the treatment of spermatogenic dysfunction caused by acute asthma. After 8 weeks of intervention, compared with the model group, the treatment group had significantly improved sperm motility (P < 0.05). There were significant differences in TNF, IL6, and INS proteins in the treatment group, and the HE staining of testicular tissue structure in the treatment group was significantly improved. CONCLUSION: JWSJP can improve the abnormal sperm motility induced by asthma, and its mechanism may be related to the expression of related proteins and mRNA of TNF, IL6, and INS. Dove 2021-03-19 /pmc/articles/PMC7989703/ /pubmed/33776422 http://dx.doi.org/10.2147/DDDT.S296901 Text en © 2021 Wang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Ji-sheng
Gong, Xue-feng
Feng, Jun-long
Li, Hai-song
Li, Xiao
Deng, Sheng
Ren, Pei-zhong
Wang, Jia-mei
Lv, Ming-sheng
Jin, Rui-feng
Chen, Qiu-yi
Wang, Bin
Cui, Hong-sheng
Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title_full Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title_fullStr Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title_full_unstemmed Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title_short Study on the Mechanism of Jiawei Shengjiang Powder in Improving Male Asthma-Induced Asthenospermia Based on Network Pharmacology and Bioinformatics
title_sort study on the mechanism of jiawei shengjiang powder in improving male asthma-induced asthenospermia based on network pharmacology and bioinformatics
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989703/
https://www.ncbi.nlm.nih.gov/pubmed/33776422
http://dx.doi.org/10.2147/DDDT.S296901
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