Cargando…

Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing

BACKGROUND: Colposcopy was referred in cases with severe abnormalities in co-testing. Although p16/Ki67 dual staining reduced the referral rate, its sensitivity and specificity need to be enhanced. METHODS: The expressions of p16, Ki-67, SMAD3, YAP1, RELA were evaluated in the colposcopy referral po...

Descripción completa

Detalles Bibliográficos
Autores principales: Huo, Xiao, Sun, Hengzi, Cao, Dongyan, Yang, Jiaxin, Peng, Peng, Kong, Linghua, Chen, Fei, Shen, Keng, Li, Shuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989978/
https://www.ncbi.nlm.nih.gov/pubmed/33776454
http://dx.doi.org/10.2147/OTT.S300269
_version_ 1783668998643122176
author Huo, Xiao
Sun, Hengzi
Cao, Dongyan
Yang, Jiaxin
Peng, Peng
Kong, Linghua
Chen, Fei
Shen, Keng
Li, Shuhong
author_facet Huo, Xiao
Sun, Hengzi
Cao, Dongyan
Yang, Jiaxin
Peng, Peng
Kong, Linghua
Chen, Fei
Shen, Keng
Li, Shuhong
author_sort Huo, Xiao
collection PubMed
description BACKGROUND: Colposcopy was referred in cases with severe abnormalities in co-testing. Although p16/Ki67 dual staining reduced the referral rate, its sensitivity and specificity need to be enhanced. METHODS: The expressions of p16, Ki-67, SMAD3, YAP1, RELA were evaluated in the colposcopy referral population. The inclusion criteria included 30–60 years and diagnosed with HPV16/18-positive, other HR-HPV-positive with ASCUS, LSIL, AGC (atypical glandular cell) in co-testing. Colposcopies, endocervical curettages of cervical biopsies were also collected. Cases were excluded if there were no biopsies, if the interval between a cervical screening test and biopsies was more than 6 months, or if insufficient tissue was available as a formalin-fixed paraffin-embedded block. The pathology was independently reviewed by two pathologists. Discrepant interpretations were adjudicated by a third pathologist. RESULTS: In total, 1194 of 1273 cases who were referred to colposcopy were evaluated in the present study. The sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 62.1% and 89.5%, respectively. p16+ combined with YAP1+ and/or RELA+ provided a sensitivity and specificity of 70.9% and 89.5%, respectively, while 72.8% and 86.4% were achieved by p16+ combined with YAP1+ and/or SMAD3+ and/or RELA+. In HPV16/18+ and LSIL subgroups, the sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 67.7% and 87.6%, respectively, for the former group and 58.6%, 88.8%, respectively, for the latter group. p16+, YAP1+/RELA+ showed a better performance for predicting CIN2+ with a better sensitivity and considerable specificity in the other HPV+ combined with ASCUS group than were achieved by p16+ combined with Ki-67+. RELA+ and the combination of p16 and RELA/YAP1 also provided the Max AUC area. CONCLUSION: Our study shows that RELA and the combination of p16 and RELA/YAP1 achieved better sensitivity and specificity for detecting morphologically CIN2+ lesions.
format Online
Article
Text
id pubmed-7989978
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-79899782021-03-25 Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing Huo, Xiao Sun, Hengzi Cao, Dongyan Yang, Jiaxin Peng, Peng Kong, Linghua Chen, Fei Shen, Keng Li, Shuhong Onco Targets Ther Original Research BACKGROUND: Colposcopy was referred in cases with severe abnormalities in co-testing. Although p16/Ki67 dual staining reduced the referral rate, its sensitivity and specificity need to be enhanced. METHODS: The expressions of p16, Ki-67, SMAD3, YAP1, RELA were evaluated in the colposcopy referral population. The inclusion criteria included 30–60 years and diagnosed with HPV16/18-positive, other HR-HPV-positive with ASCUS, LSIL, AGC (atypical glandular cell) in co-testing. Colposcopies, endocervical curettages of cervical biopsies were also collected. Cases were excluded if there were no biopsies, if the interval between a cervical screening test and biopsies was more than 6 months, or if insufficient tissue was available as a formalin-fixed paraffin-embedded block. The pathology was independently reviewed by two pathologists. Discrepant interpretations were adjudicated by a third pathologist. RESULTS: In total, 1194 of 1273 cases who were referred to colposcopy were evaluated in the present study. The sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 62.1% and 89.5%, respectively. p16+ combined with YAP1+ and/or RELA+ provided a sensitivity and specificity of 70.9% and 89.5%, respectively, while 72.8% and 86.4% were achieved by p16+ combined with YAP1+ and/or SMAD3+ and/or RELA+. In HPV16/18+ and LSIL subgroups, the sensitivity and specificity of p16+ combined with Ki-67+ for predicting CIN2+ were 67.7% and 87.6%, respectively, for the former group and 58.6%, 88.8%, respectively, for the latter group. p16+, YAP1+/RELA+ showed a better performance for predicting CIN2+ with a better sensitivity and considerable specificity in the other HPV+ combined with ASCUS group than were achieved by p16+ combined with Ki-67+. RELA+ and the combination of p16 and RELA/YAP1 also provided the Max AUC area. CONCLUSION: Our study shows that RELA and the combination of p16 and RELA/YAP1 achieved better sensitivity and specificity for detecting morphologically CIN2+ lesions. Dove 2021-03-19 /pmc/articles/PMC7989978/ /pubmed/33776454 http://dx.doi.org/10.2147/OTT.S300269 Text en © 2021 Huo et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Huo, Xiao
Sun, Hengzi
Cao, Dongyan
Yang, Jiaxin
Peng, Peng
Kong, Linghua
Chen, Fei
Shen, Keng
Li, Shuhong
Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title_full Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title_fullStr Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title_full_unstemmed Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title_short Evaluation of Cervical High-Grade Squamous Intraepithelial Lesions-Correlated Markers as Triage Strategy for Colposcopy After Co-Testing
title_sort evaluation of cervical high-grade squamous intraepithelial lesions-correlated markers as triage strategy for colposcopy after co-testing
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989978/
https://www.ncbi.nlm.nih.gov/pubmed/33776454
http://dx.doi.org/10.2147/OTT.S300269
work_keys_str_mv AT huoxiao evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT sunhengzi evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT caodongyan evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT yangjiaxin evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT pengpeng evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT konglinghua evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT chenfei evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT shenkeng evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting
AT lishuhong evaluationofcervicalhighgradesquamousintraepitheliallesionscorrelatedmarkersastriagestrategyforcolposcopyaftercotesting