Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones

An unprecedented number of viruses have been discovered by leveraging advances in high-throughput sequencing. Infectious clone technology is a universal approach that facilitates the study of biology and role in disease of viruses. In recent years homology-based cloning methods such as Gibson assemb...

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Detalles Bibliográficos
Autores principales: Zhao, Mingmin, García, Beatriz, Gallo, Araiz, Tzanetakis, Ioannis E., Simón-Mateo, Carmen, García, Juan Antonio, Pasin, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990137/
https://www.ncbi.nlm.nih.gov/pubmed/33768973
http://dx.doi.org/10.1186/s42483-020-00077-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990137/
https://www.ncbi.nlm.nih.gov/pubmed/33768973
http://dx.doi.org/10.1186/s42483-020-00077-4

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