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Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones
An unprecedented number of viruses have been discovered by leveraging advances in high-throughput sequencing. Infectious clone technology is a universal approach that facilitates the study of biology and role in disease of viruses. In recent years homology-based cloning methods such as Gibson assemb...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990137/ https://www.ncbi.nlm.nih.gov/pubmed/33768973 http://dx.doi.org/10.1186/s42483-020-00077-4 |