Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.

Seroconversion panels are an important tool for investigating antibody responses and developing serological assays. A seroconversion panel was generated from a single SARS-CoV-2 positive plasma donor over 87 days. This seroconversion panel was tested against 6 SARS-CoV-2 antibody tests (IgG, IgM, an...

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Autores principales: Belda, Francisco, Lopez-Martinez, Monica, Torres, Nerea, Cherenzia, Robin, Crowley, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990520/
https://www.ncbi.nlm.nih.gov/pubmed/33596485
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115340
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author Belda, Francisco
Lopez-Martinez, Monica
Torres, Nerea
Cherenzia, Robin
Crowley, Michael
author_facet Belda, Francisco
Lopez-Martinez, Monica
Torres, Nerea
Cherenzia, Robin
Crowley, Michael
author_sort Belda, Francisco
collection PubMed
description Seroconversion panels are an important tool for investigating antibody responses and developing serological assays. A seroconversion panel was generated from a single SARS-CoV-2 positive plasma donor over 87 days. This seroconversion panel was tested against 6 SARS-CoV-2 antibody tests (IgG, IgM, and total Ig). All test kits utilized recombinant antigens that are specific to SARS-CoV-2. The seroconversion panel showed IgG responses for SARS-CoV-2 after day 50. IgM levels peaked on day 50 (prior to IgG) and declined in subsequent samples. This seroconversion panel is a useful tool for validation of SARS-CoV-2 antibody assays.
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spelling pubmed-79905202021-03-25 Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays. Belda, Francisco Lopez-Martinez, Monica Torres, Nerea Cherenzia, Robin Crowley, Michael Diagn Microbiol Infect Dis Article Seroconversion panels are an important tool for investigating antibody responses and developing serological assays. A seroconversion panel was generated from a single SARS-CoV-2 positive plasma donor over 87 days. This seroconversion panel was tested against 6 SARS-CoV-2 antibody tests (IgG, IgM, and total Ig). All test kits utilized recombinant antigens that are specific to SARS-CoV-2. The seroconversion panel showed IgG responses for SARS-CoV-2 after day 50. IgM levels peaked on day 50 (prior to IgG) and declined in subsequent samples. This seroconversion panel is a useful tool for validation of SARS-CoV-2 antibody assays. The Authors. Published by Elsevier Inc. 2021-06 2021-02-06 /pmc/articles/PMC7990520/ /pubmed/33596485 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115340 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Belda, Francisco
Lopez-Martinez, Monica
Torres, Nerea
Cherenzia, Robin
Crowley, Michael
Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title_full Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title_fullStr Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title_full_unstemmed Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title_short Available COVID-19 serial seroconversion panel for validation of SARS-CoV-2 antibody assays.
title_sort available covid-19 serial seroconversion panel for validation of sars-cov-2 antibody assays.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990520/
https://www.ncbi.nlm.nih.gov/pubmed/33596485
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115340
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