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Actin cables and comet tails organize mitochondrial networks in mitosis
Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. While the mechanisms coordinating the segregation of the genome are well known, the processes which ensure organelle segregation between daughter cells remain less well-unde...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990722/ https://www.ncbi.nlm.nih.gov/pubmed/33658713 http://dx.doi.org/10.1038/s41586-021-03309-5 |
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author | Moore, Andrew S. Coscia, Stephen M. Simpson, Cory L. Ortega, Fabian E. Wait, Eric C. Heddleston, John M. Nirschl, Jeffrey J. Obara, Christopher J. Guedes-Dias, Pedro Boecker, C. Alexander Chew, Teng-Leong Theriot, Julie A. Lippincott-Schwartz, Jennifer Holzbaur, Erika L.F. |
author_facet | Moore, Andrew S. Coscia, Stephen M. Simpson, Cory L. Ortega, Fabian E. Wait, Eric C. Heddleston, John M. Nirschl, Jeffrey J. Obara, Christopher J. Guedes-Dias, Pedro Boecker, C. Alexander Chew, Teng-Leong Theriot, Julie A. Lippincott-Schwartz, Jennifer Holzbaur, Erika L.F. |
author_sort | Moore, Andrew S. |
collection | PubMed |
description | Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. While the mechanisms coordinating the segregation of the genome are well known, the processes which ensure organelle segregation between daughter cells remain less well-understood(1). Here, we identify multiple actin assemblies that play distinct but complementary roles in mitochondrial organization and inheritance in mitosis. First, we find a dense meshwork of subcortical actin cables assembled throughout the mitotic cytoplasm. This network scaffolds the endoplasmic reticulum and organizes three-dimensional mitochondrial positioning to ensure the equal segregation of mitochondrial mass at cytokinesis. Second, we identify a dynamic wave of actin filaments reversibly assembling on the surface of mitochondria through mitosis. Mitochondria sampled by this wave are enveloped within actin clouds that can spontaneously break symmetry to form elongated comet tails. Mitochondrial comet tails promote randomly directed bursts of movement that shuffle mitochondrial position within the mother cell to randomize inheritance of healthy and damaged mitochondria between daughter cells. Thus, parallel mechanisms mediated by the actin cytoskeleton ensure both equal and random inheritance of mitochondria in symmetrically dividing cells. |
format | Online Article Text |
id | pubmed-7990722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-79907222021-09-03 Actin cables and comet tails organize mitochondrial networks in mitosis Moore, Andrew S. Coscia, Stephen M. Simpson, Cory L. Ortega, Fabian E. Wait, Eric C. Heddleston, John M. Nirschl, Jeffrey J. Obara, Christopher J. Guedes-Dias, Pedro Boecker, C. Alexander Chew, Teng-Leong Theriot, Julie A. Lippincott-Schwartz, Jennifer Holzbaur, Erika L.F. Nature Article Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. While the mechanisms coordinating the segregation of the genome are well known, the processes which ensure organelle segregation between daughter cells remain less well-understood(1). Here, we identify multiple actin assemblies that play distinct but complementary roles in mitochondrial organization and inheritance in mitosis. First, we find a dense meshwork of subcortical actin cables assembled throughout the mitotic cytoplasm. This network scaffolds the endoplasmic reticulum and organizes three-dimensional mitochondrial positioning to ensure the equal segregation of mitochondrial mass at cytokinesis. Second, we identify a dynamic wave of actin filaments reversibly assembling on the surface of mitochondria through mitosis. Mitochondria sampled by this wave are enveloped within actin clouds that can spontaneously break symmetry to form elongated comet tails. Mitochondrial comet tails promote randomly directed bursts of movement that shuffle mitochondrial position within the mother cell to randomize inheritance of healthy and damaged mitochondria between daughter cells. Thus, parallel mechanisms mediated by the actin cytoskeleton ensure both equal and random inheritance of mitochondria in symmetrically dividing cells. 2021-03-03 2021-03 /pmc/articles/PMC7990722/ /pubmed/33658713 http://dx.doi.org/10.1038/s41586-021-03309-5 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Moore, Andrew S. Coscia, Stephen M. Simpson, Cory L. Ortega, Fabian E. Wait, Eric C. Heddleston, John M. Nirschl, Jeffrey J. Obara, Christopher J. Guedes-Dias, Pedro Boecker, C. Alexander Chew, Teng-Leong Theriot, Julie A. Lippincott-Schwartz, Jennifer Holzbaur, Erika L.F. Actin cables and comet tails organize mitochondrial networks in mitosis |
title | Actin cables and comet tails organize mitochondrial networks in mitosis |
title_full | Actin cables and comet tails organize mitochondrial networks in mitosis |
title_fullStr | Actin cables and comet tails organize mitochondrial networks in mitosis |
title_full_unstemmed | Actin cables and comet tails organize mitochondrial networks in mitosis |
title_short | Actin cables and comet tails organize mitochondrial networks in mitosis |
title_sort | actin cables and comet tails organize mitochondrial networks in mitosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7990722/ https://www.ncbi.nlm.nih.gov/pubmed/33658713 http://dx.doi.org/10.1038/s41586-021-03309-5 |
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