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Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus
BACKGROUND: In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991009/ https://www.ncbi.nlm.nih.gov/pubmed/33761027 http://dx.doi.org/10.1186/s43141-021-00143-0 |
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author | Sadat, Md. Abu Ullah, Md. Wali Bashar, Kazi Khayrul Hossen, Quazi Md. Mosaddeque Tareq, Md. Zablul Islam, Md. Shahidul |
author_facet | Sadat, Md. Abu Ullah, Md. Wali Bashar, Kazi Khayrul Hossen, Quazi Md. Mosaddeque Tareq, Md. Zablul Islam, Md. Shahidul |
author_sort | Sadat, Md. Abu |
collection | PubMed |
description | BACKGROUND: In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in the Macrophomina phaseolina fungus. RESULTS: In this research, in silico studies were done to understand the distribution of F-box proteins in pathogenic fungi including Macrophomina phaseolina fungus. Genome-wide analysis indicates that M. phaseolina fungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified from Puccinia graminis, Colletotrichum graminicola, Ustilago maydis, and Phytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development. CONCLUSION: This genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development of M. phaseolina in the host plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00143-0. |
format | Online Article Text |
id | pubmed-7991009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-79910092021-04-12 Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus Sadat, Md. Abu Ullah, Md. Wali Bashar, Kazi Khayrul Hossen, Quazi Md. Mosaddeque Tareq, Md. Zablul Islam, Md. Shahidul J Genet Eng Biotechnol Research BACKGROUND: In fungi, like other eukaryotes, protein turnover is an important cellular process for the controlling of various cellular functions. The ubiquitin-proteasome pathway degrades some selected intracellular proteins and F-box proteins are one of the important components controlling protein degradation. F-box proteins are well studied in different model plants however, their functions in the fungi are not clear yet. This study aimed to identify the genes involved in protein degradation for disease development in the Macrophomina phaseolina fungus. RESULTS: In this research, in silico studies were done to understand the distribution of F-box proteins in pathogenic fungi including Macrophomina phaseolina fungus. Genome-wide analysis indicates that M. phaseolina fungus contained thirty-one F-box proteins throughout its chromosomes. In addition, there are 17, 37, 16, and 21 F-box proteins have been identified from Puccinia graminis, Colletotrichum graminicola, Ustilago maydis, and Phytophthora infestans, respectively. Analyses revealed that selective fungal genomes contain several additional functional domains along with F-box domain. Sequence alignment showed the substitution of amino acid in several F-box proteins; however, gene duplication was not found among these proteins. Phylogenetic analysis revealed that F-box proteins having similar functional domain was highly diverse form each other showing the possibility of various function. Analysis also found that MPH_00568 and MPH_05531 were closely related to rice blast fungus F-box protein MGG_00768 and MGG_13065, respectively, may play an important role for blast disease development. CONCLUSION: This genome-wide analysis of F-box proteins will be useful for characterization of candidate F-box proteins to understand the molecular mechanisms leading to disease development of M. phaseolina in the host plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00143-0. Springer Berlin Heidelberg 2021-03-24 /pmc/articles/PMC7991009/ /pubmed/33761027 http://dx.doi.org/10.1186/s43141-021-00143-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Sadat, Md. Abu Ullah, Md. Wali Bashar, Kazi Khayrul Hossen, Quazi Md. Mosaddeque Tareq, Md. Zablul Islam, Md. Shahidul Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title | Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title_full | Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title_fullStr | Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title_full_unstemmed | Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title_short | Genome-wide identification of F-box proteins in Macrophomina phaseolina and comparison with other fungus |
title_sort | genome-wide identification of f-box proteins in macrophomina phaseolina and comparison with other fungus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991009/ https://www.ncbi.nlm.nih.gov/pubmed/33761027 http://dx.doi.org/10.1186/s43141-021-00143-0 |
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