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miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction

Current tests available to diagnose fetal hypoxia in-utero lack sensitivity thus failing to identify many fetuses at risk. Emerging evidence suggests that microRNAs derived from the placenta circulate in the maternal blood during pregnancy and may be used as non-invasive biomarkers for pregnancy com...

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Autores principales: Tagliaferri, Salvatore, Cepparulo, Pasquale, Vinciguerra, Antonio, Campanile, Marta, Esposito, Giuseppina, Maruotti, Giuseppe Maria, Zullo, Fulvio, Annunziato, Lucio, Pignataro, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991078/
https://www.ncbi.nlm.nih.gov/pubmed/33777862
http://dx.doi.org/10.3389/fped.2021.611112
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author Tagliaferri, Salvatore
Cepparulo, Pasquale
Vinciguerra, Antonio
Campanile, Marta
Esposito, Giuseppina
Maruotti, Giuseppe Maria
Zullo, Fulvio
Annunziato, Lucio
Pignataro, Giuseppe
author_facet Tagliaferri, Salvatore
Cepparulo, Pasquale
Vinciguerra, Antonio
Campanile, Marta
Esposito, Giuseppina
Maruotti, Giuseppe Maria
Zullo, Fulvio
Annunziato, Lucio
Pignataro, Giuseppe
author_sort Tagliaferri, Salvatore
collection PubMed
description Current tests available to diagnose fetal hypoxia in-utero lack sensitivity thus failing to identify many fetuses at risk. Emerging evidence suggests that microRNAs derived from the placenta circulate in the maternal blood during pregnancy and may be used as non-invasive biomarkers for pregnancy complications. With the intent to identify putative markers of fetal growth restriction (FGR) and new therapeutic druggable targets, we examined, in maternal blood samples, the expression of a group of microRNAs, known to be regulated by hypoxia. The expression of microRNAs was evaluated in maternal plasma samples collected from (1) women carrying a preterm FGR fetus (FGR group) or (2) women with an appropriately grown fetus matched at the same gestational age (Control group). To discriminate between early- and late-onset FGR, the study population was divided into two subgroups according to the gestational age at delivery. Four microRNAs were identified as possible candidates for the diagnosis of FGR: miR-16-5p, miR-103-3p, miR-107-3p, and miR-27b-3p. All four selected miRNAs, measured by RT-PCR, resulted upregulated in FGR blood samples before the 32nd week of gestation. By contrast, miRNA103-3p and miRNA107-3p, analyzed between the 32nd and 37th week of gestation, showed lower expression in the FGR group compared to aged matched controls. Our results showed that measurement of miRNAs in maternal blood may form the basis for a future diagnostic test to determine the degree of fetal hypoxia in FGR, thus allowing the start of appropriate therapeutic interventions to alleviate the burden of this disease.
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spelling pubmed-79910782021-03-26 miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction Tagliaferri, Salvatore Cepparulo, Pasquale Vinciguerra, Antonio Campanile, Marta Esposito, Giuseppina Maruotti, Giuseppe Maria Zullo, Fulvio Annunziato, Lucio Pignataro, Giuseppe Front Pediatr Pediatrics Current tests available to diagnose fetal hypoxia in-utero lack sensitivity thus failing to identify many fetuses at risk. Emerging evidence suggests that microRNAs derived from the placenta circulate in the maternal blood during pregnancy and may be used as non-invasive biomarkers for pregnancy complications. With the intent to identify putative markers of fetal growth restriction (FGR) and new therapeutic druggable targets, we examined, in maternal blood samples, the expression of a group of microRNAs, known to be regulated by hypoxia. The expression of microRNAs was evaluated in maternal plasma samples collected from (1) women carrying a preterm FGR fetus (FGR group) or (2) women with an appropriately grown fetus matched at the same gestational age (Control group). To discriminate between early- and late-onset FGR, the study population was divided into two subgroups according to the gestational age at delivery. Four microRNAs were identified as possible candidates for the diagnosis of FGR: miR-16-5p, miR-103-3p, miR-107-3p, and miR-27b-3p. All four selected miRNAs, measured by RT-PCR, resulted upregulated in FGR blood samples before the 32nd week of gestation. By contrast, miRNA103-3p and miRNA107-3p, analyzed between the 32nd and 37th week of gestation, showed lower expression in the FGR group compared to aged matched controls. Our results showed that measurement of miRNAs in maternal blood may form the basis for a future diagnostic test to determine the degree of fetal hypoxia in FGR, thus allowing the start of appropriate therapeutic interventions to alleviate the burden of this disease. Frontiers Media S.A. 2021-03-11 /pmc/articles/PMC7991078/ /pubmed/33777862 http://dx.doi.org/10.3389/fped.2021.611112 Text en Copyright © 2021 Tagliaferri, Cepparulo, Vinciguerra, Campanile, Esposito, Maruotti, Zullo, Annunziato and Pignataro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Tagliaferri, Salvatore
Cepparulo, Pasquale
Vinciguerra, Antonio
Campanile, Marta
Esposito, Giuseppina
Maruotti, Giuseppe Maria
Zullo, Fulvio
Annunziato, Lucio
Pignataro, Giuseppe
miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title_full miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title_fullStr miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title_full_unstemmed miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title_short miR-16-5p, miR-103-3p, and miR-27b-3p as Early Peripheral Biomarkers of Fetal Growth Restriction
title_sort mir-16-5p, mir-103-3p, and mir-27b-3p as early peripheral biomarkers of fetal growth restriction
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991078/
https://www.ncbi.nlm.nih.gov/pubmed/33777862
http://dx.doi.org/10.3389/fped.2021.611112
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