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Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer

Junctional adhesion molecule-like protein (JAML), a newly discovered junctional adhesion molecule (JAM), mediates the adhesion and migration processes of various immune cells and endothelial/epithelial cells, ultimately regulating inflammation reaction. However, its role in tumors remains to be dete...

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Autores principales: Fang, Yuying, Yang, Jianmin, Zu, Guohong, Cong, Changsheng, Liu, Shuai, Xue, Fei, Ma, Shuzhen, Liu, Jie, Sun, Yuping, Sun, Meili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991718/
https://www.ncbi.nlm.nih.gov/pubmed/33777731
http://dx.doi.org/10.3389/fonc.2021.565676
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author Fang, Yuying
Yang, Jianmin
Zu, Guohong
Cong, Changsheng
Liu, Shuai
Xue, Fei
Ma, Shuzhen
Liu, Jie
Sun, Yuping
Sun, Meili
author_facet Fang, Yuying
Yang, Jianmin
Zu, Guohong
Cong, Changsheng
Liu, Shuai
Xue, Fei
Ma, Shuzhen
Liu, Jie
Sun, Yuping
Sun, Meili
author_sort Fang, Yuying
collection PubMed
description Junctional adhesion molecule-like protein (JAML), a newly discovered junctional adhesion molecule (JAM), mediates the adhesion and migration processes of various immune cells and endothelial/epithelial cells, ultimately regulating inflammation reaction. However, its role in tumors remains to be determined. The expression of JAML was examined in gastric cancer (GC) and peritumoral tissues from 63 patients. The relationship between JAML expression and clinical characteristics was also observed. In vitro, GC cell migration and proliferation were assessed by wound healing assay, transwell migration assay and EdU incorporation assay. Immunohistochemical staining results showed that JAML expression level was higher in GC tissues than in peritumoral tissues. High expression of JAML in cancer tissues was associated with worse cell differentiation, local lymph node involvement, deep infiltration, and advanced stage. In vitro, we found that JAML silencing inhibited GC cell migration and proliferation, while JAML overexpression promoted GC cell migration and proliferation, partially via p38 signaling. Taken together, our study revealed a critical role for JAML to promote GC cell migration and proliferation. JAML might be a novel diagnostic biomarker and therapeutic target for GC.
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spelling pubmed-79917182021-03-26 Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer Fang, Yuying Yang, Jianmin Zu, Guohong Cong, Changsheng Liu, Shuai Xue, Fei Ma, Shuzhen Liu, Jie Sun, Yuping Sun, Meili Front Oncol Oncology Junctional adhesion molecule-like protein (JAML), a newly discovered junctional adhesion molecule (JAM), mediates the adhesion and migration processes of various immune cells and endothelial/epithelial cells, ultimately regulating inflammation reaction. However, its role in tumors remains to be determined. The expression of JAML was examined in gastric cancer (GC) and peritumoral tissues from 63 patients. The relationship between JAML expression and clinical characteristics was also observed. In vitro, GC cell migration and proliferation were assessed by wound healing assay, transwell migration assay and EdU incorporation assay. Immunohistochemical staining results showed that JAML expression level was higher in GC tissues than in peritumoral tissues. High expression of JAML in cancer tissues was associated with worse cell differentiation, local lymph node involvement, deep infiltration, and advanced stage. In vitro, we found that JAML silencing inhibited GC cell migration and proliferation, while JAML overexpression promoted GC cell migration and proliferation, partially via p38 signaling. Taken together, our study revealed a critical role for JAML to promote GC cell migration and proliferation. JAML might be a novel diagnostic biomarker and therapeutic target for GC. Frontiers Media S.A. 2021-03-11 /pmc/articles/PMC7991718/ /pubmed/33777731 http://dx.doi.org/10.3389/fonc.2021.565676 Text en Copyright © 2021 Fang, Yang, Zu, Cong, Liu, Xue, Ma, Liu, Sun and Sun http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Fang, Yuying
Yang, Jianmin
Zu, Guohong
Cong, Changsheng
Liu, Shuai
Xue, Fei
Ma, Shuzhen
Liu, Jie
Sun, Yuping
Sun, Meili
Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title_full Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title_fullStr Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title_full_unstemmed Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title_short Junctional Adhesion Molecule-Like Protein Promotes Tumor Progression and Metastasis via p38 Signaling Pathway in Gastric Cancer
title_sort junctional adhesion molecule-like protein promotes tumor progression and metastasis via p38 signaling pathway in gastric cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991718/
https://www.ncbi.nlm.nih.gov/pubmed/33777731
http://dx.doi.org/10.3389/fonc.2021.565676
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