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The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage

The successful elimination of bulky DNA damages via the nucleotide excision repair (NER) system is largely determined by the damage recognition step. This step consists of primary recognition and verification of the damage. The TFIIH helicase XPD plays a key role in the verification step during NER....

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Autores principales: Petruseva, Irina, Naumenko, Natalia, Kuper, Jochen, Anarbaev, Rashid, Kappenberger, Jeannette, Kisker, Caroline, Lavrik, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991749/
https://www.ncbi.nlm.nih.gov/pubmed/33777931
http://dx.doi.org/10.3389/fcell.2021.617160
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author Petruseva, Irina
Naumenko, Natalia
Kuper, Jochen
Anarbaev, Rashid
Kappenberger, Jeannette
Kisker, Caroline
Lavrik, Olga
author_facet Petruseva, Irina
Naumenko, Natalia
Kuper, Jochen
Anarbaev, Rashid
Kappenberger, Jeannette
Kisker, Caroline
Lavrik, Olga
author_sort Petruseva, Irina
collection PubMed
description The successful elimination of bulky DNA damages via the nucleotide excision repair (NER) system is largely determined by the damage recognition step. This step consists of primary recognition and verification of the damage. The TFIIH helicase XPD plays a key role in the verification step during NER. To date, the mechanism of damage verification is not sufficiently understood and requires further detailed research. This study is a systematic investigation of the interaction of ctXPD (Chaetomium thermophilum) as well as ctXPD-ctp44 with model DNAs, which contain structurally different bulky lesions with previously estimated NER repair efficiencies. We have used ATPase and DNA binding studies to assess the interaction of ctXPD with damaged DNA. The result of the analysis of ctXPD-ctp44 binding to DNA containing fluorescent and photoactivatable lesions demonstrates the relationship between the affinity of XPD for DNAs containing bulky damages and the ability of the NER system to eliminate the damage. Photo-cross-linking of ctXPD with DNA probes containing repairable and unrepairable photoactivatable damages reveals differences in the DNA interaction efficiency in the presence and absence of ctp44. In general, the results obtained indicate the ability of ctXPD-ctp44 to interact with a damage and suggest a significant role for ctp44 subunit in the verification process.
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spelling pubmed-79917492021-03-26 The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage Petruseva, Irina Naumenko, Natalia Kuper, Jochen Anarbaev, Rashid Kappenberger, Jeannette Kisker, Caroline Lavrik, Olga Front Cell Dev Biol Cell and Developmental Biology The successful elimination of bulky DNA damages via the nucleotide excision repair (NER) system is largely determined by the damage recognition step. This step consists of primary recognition and verification of the damage. The TFIIH helicase XPD plays a key role in the verification step during NER. To date, the mechanism of damage verification is not sufficiently understood and requires further detailed research. This study is a systematic investigation of the interaction of ctXPD (Chaetomium thermophilum) as well as ctXPD-ctp44 with model DNAs, which contain structurally different bulky lesions with previously estimated NER repair efficiencies. We have used ATPase and DNA binding studies to assess the interaction of ctXPD with damaged DNA. The result of the analysis of ctXPD-ctp44 binding to DNA containing fluorescent and photoactivatable lesions demonstrates the relationship between the affinity of XPD for DNAs containing bulky damages and the ability of the NER system to eliminate the damage. Photo-cross-linking of ctXPD with DNA probes containing repairable and unrepairable photoactivatable damages reveals differences in the DNA interaction efficiency in the presence and absence of ctp44. In general, the results obtained indicate the ability of ctXPD-ctp44 to interact with a damage and suggest a significant role for ctp44 subunit in the verification process. Frontiers Media S.A. 2021-03-11 /pmc/articles/PMC7991749/ /pubmed/33777931 http://dx.doi.org/10.3389/fcell.2021.617160 Text en Copyright © 2021 Petruseva, Naumenko, Kuper, Anarbaev, Kappenberger, Kisker and Lavrik. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Petruseva, Irina
Naumenko, Natalia
Kuper, Jochen
Anarbaev, Rashid
Kappenberger, Jeannette
Kisker, Caroline
Lavrik, Olga
The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title_full The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title_fullStr The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title_full_unstemmed The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title_short The Interaction Efficiency of XPD-p44 With Bulky DNA Damages Depends on the Structure of the Damage
title_sort interaction efficiency of xpd-p44 with bulky dna damages depends on the structure of the damage
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991749/
https://www.ncbi.nlm.nih.gov/pubmed/33777931
http://dx.doi.org/10.3389/fcell.2021.617160
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