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Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients
Objective: To study the correlation between the cycle threshold (CT) of reverse transcription–polymerase chain reaction (RT–PCR) test in confirmed COVID-19 patients and the severity of disease. Background: RT–PCR test is a standard method for the diagnosis of severe acute respiratory syndrome corona...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Jaypee Brothers Medical Publishers
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991767/ https://www.ncbi.nlm.nih.gov/pubmed/33790515 http://dx.doi.org/10.5005/jp-journals-10071-23765 |
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author | Rajyalakshmi, B Samavedam, Srinivas Reddy, P Ramakrishna Aluru, Narmada |
author_facet | Rajyalakshmi, B Samavedam, Srinivas Reddy, P Ramakrishna Aluru, Narmada |
author_sort | Rajyalakshmi, B |
collection | PubMed |
description | Objective: To study the correlation between the cycle threshold (CT) of reverse transcription–polymerase chain reaction (RT–PCR) test in confirmed COVID-19 patients and the severity of disease. Background: RT–PCR test is a standard method for the diagnosis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections. This test is based upon the amplification of the fluorescent signal. The number of cycles that the fluorescent signal undergoes to reach the threshold is called “cycle threshold.” It is inversely related to the nucleic acid content of the sample. Patients and methods: This is a single-centered, retrospective observational study. We have included a total of 192 patients. SARS-CoV-2 infection was confirmed by the RT–PCR test. Entire data have been collected from the electronic medical records. The primary outcome was 28-day mortality, whereas the secondary outcomes were intensive care unit (ICU) admission, invasive ventilation, acute kidney injury, renal replacement therapy (RRT), shock, and COVID-19 reporting and data system (CO-RADS) score on high-resolution computed tomography of the chest, total length of stay in the hospital, and the number of ICU days and ventilator days. Results: We have calculated the mean CT value for all groups and calculated the p-value for statistical significance. For the total length of stay in the hospital and the number of ICU days and ventilator days, we applied the Pearson correlation coefficient. The p-value was statistically significant for mortality, ICU admission, and shock groups. The CT values and the length of ICU stay were inversely correlated with the statistically significant p-value. Conclusion: Low CT value is associated with increased ICU admission, high mortality, shock, and increased length of ICU stay. How to cite this article: Rajyalakshmi B, Samavedam S, Reddy PR, Aluru N. Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients. Indian J Crit Care Med 2021;25(3):322–326. |
format | Online Article Text |
id | pubmed-7991767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Jaypee Brothers Medical Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-79917672021-03-30 Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients Rajyalakshmi, B Samavedam, Srinivas Reddy, P Ramakrishna Aluru, Narmada Indian J Crit Care Med Original Research Objective: To study the correlation between the cycle threshold (CT) of reverse transcription–polymerase chain reaction (RT–PCR) test in confirmed COVID-19 patients and the severity of disease. Background: RT–PCR test is a standard method for the diagnosis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections. This test is based upon the amplification of the fluorescent signal. The number of cycles that the fluorescent signal undergoes to reach the threshold is called “cycle threshold.” It is inversely related to the nucleic acid content of the sample. Patients and methods: This is a single-centered, retrospective observational study. We have included a total of 192 patients. SARS-CoV-2 infection was confirmed by the RT–PCR test. Entire data have been collected from the electronic medical records. The primary outcome was 28-day mortality, whereas the secondary outcomes were intensive care unit (ICU) admission, invasive ventilation, acute kidney injury, renal replacement therapy (RRT), shock, and COVID-19 reporting and data system (CO-RADS) score on high-resolution computed tomography of the chest, total length of stay in the hospital, and the number of ICU days and ventilator days. Results: We have calculated the mean CT value for all groups and calculated the p-value for statistical significance. For the total length of stay in the hospital and the number of ICU days and ventilator days, we applied the Pearson correlation coefficient. The p-value was statistically significant for mortality, ICU admission, and shock groups. The CT values and the length of ICU stay were inversely correlated with the statistically significant p-value. Conclusion: Low CT value is associated with increased ICU admission, high mortality, shock, and increased length of ICU stay. How to cite this article: Rajyalakshmi B, Samavedam S, Reddy PR, Aluru N. Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients. Indian J Crit Care Med 2021;25(3):322–326. Jaypee Brothers Medical Publishers 2021-03 /pmc/articles/PMC7991767/ /pubmed/33790515 http://dx.doi.org/10.5005/jp-journals-10071-23765 Text en Copyright © 2021; Jaypee Brothers Medical Publishers (P) Ltd. © Jaypee Brothers Medical Publishers. 2021 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and non-commercial reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Original Research Rajyalakshmi, B Samavedam, Srinivas Reddy, P Ramakrishna Aluru, Narmada Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title | Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title_full | Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title_fullStr | Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title_full_unstemmed | Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title_short | Prognostic Value of “Cycle Threshold” in Confirmed COVID-19 Patients |
title_sort | prognostic value of “cycle threshold” in confirmed covid-19 patients |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991767/ https://www.ncbi.nlm.nih.gov/pubmed/33790515 http://dx.doi.org/10.5005/jp-journals-10071-23765 |
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