Long non-coding RNA LNC_000641 regulates pseudorabies virus replication

Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant...

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Autores principales: Fang, Linlin, Gao, Yanni, Liu, Xing, Bai, Juan, Jiang, Ping, Wang, XianWei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992786/
https://www.ncbi.nlm.nih.gov/pubmed/33766129
http://dx.doi.org/10.1186/s13567-021-00922-0
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author Fang, Linlin
Gao, Yanni
Liu, Xing
Bai, Juan
Jiang, Ping
Wang, XianWei
author_facet Fang, Linlin
Gao, Yanni
Liu, Xing
Bai, Juan
Jiang, Ping
Wang, XianWei
author_sort Fang, Linlin
collection PubMed
description Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.
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spelling pubmed-79927862021-03-25 Long non-coding RNA LNC_000641 regulates pseudorabies virus replication Fang, Linlin Gao, Yanni Liu, Xing Bai, Juan Jiang, Ping Wang, XianWei Vet Res Research Article Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection. BioMed Central 2021-03-25 2021 /pmc/articles/PMC7992786/ /pubmed/33766129 http://dx.doi.org/10.1186/s13567-021-00922-0 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Fang, Linlin
Gao, Yanni
Liu, Xing
Bai, Juan
Jiang, Ping
Wang, XianWei
Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title_full Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title_fullStr Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title_full_unstemmed Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title_short Long non-coding RNA LNC_000641 regulates pseudorabies virus replication
title_sort long non-coding rna lnc_000641 regulates pseudorabies virus replication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992786/
https://www.ncbi.nlm.nih.gov/pubmed/33766129
http://dx.doi.org/10.1186/s13567-021-00922-0
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AT baijuan longnoncodingrnalnc000641regulatespseudorabiesvirusreplication
AT jiangping longnoncodingrnalnc000641regulatespseudorabiesvirusreplication
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