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Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients

BACKGROUND: Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macroph...

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Autores principales: Mohd Idrus, Fatin Najiah, Ahmad, Nurul Shuhadah, Hoe, Chee Hock, Azlan, Maryam, Norfuad, Farisha Alia, Yusof, Zurkurnai, Wan Isa, Wan Yus Haniff, Mohamed Ali, Akbar Ali, Yvonne-Tee, Get Bee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992933/
https://www.ncbi.nlm.nih.gov/pubmed/33761885
http://dx.doi.org/10.1186/s12865-021-00410-2
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author Mohd Idrus, Fatin Najiah
Ahmad, Nurul Shuhadah
Hoe, Chee Hock
Azlan, Maryam
Norfuad, Farisha Alia
Yusof, Zurkurnai
Wan Isa, Wan Yus Haniff
Mohamed Ali, Akbar Ali
Yvonne-Tee, Get Bee
author_facet Mohd Idrus, Fatin Najiah
Ahmad, Nurul Shuhadah
Hoe, Chee Hock
Azlan, Maryam
Norfuad, Farisha Alia
Yusof, Zurkurnai
Wan Isa, Wan Yus Haniff
Mohamed Ali, Akbar Ali
Yvonne-Tee, Get Bee
author_sort Mohd Idrus, Fatin Najiah
collection PubMed
description BACKGROUND: Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown. OBJECTIVE: This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes. METHODS: A total of 14 (n) CAD patients were recruited and subsequently grouped into “no apparent CAD”, “non-obstructive CAD” and “obstructive CAD” according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86(+)/CD80(+) for M1 and CD206(+)/CD200R(+) for M2) and measurement of MerTK expression were performed using flow cytometry. RESULTS: Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040]. CONCLUSION: Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00410-2.
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spelling pubmed-79929332021-03-25 Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients Mohd Idrus, Fatin Najiah Ahmad, Nurul Shuhadah Hoe, Chee Hock Azlan, Maryam Norfuad, Farisha Alia Yusof, Zurkurnai Wan Isa, Wan Yus Haniff Mohamed Ali, Akbar Ali Yvonne-Tee, Get Bee BMC Immunol Research Article BACKGROUND: Differential polarization of macrophage into M1 and M2 mediates atherosclerotic plaque clearance through efferocytosis. Higher expression of Mer proto-oncogene tyrosine kinase (MerTK) on M2 macrophage helps in maintaining macrophage efferocytic efficiency. In healthy individuals, macrophage polarization into M1 and M2 occurs in tissues in concomitance with the acquisition of functional phenotypes depending on specific microenvironment stimuli. However, whether the macrophage differential polarization and MerTK expression vary in coronary artery disease (CAD) patients remain unknown. OBJECTIVE: This study aimed to elucidate the polarization of M1 and M2 macrophage from CAD patients as well as to investigate the expression of MerTK in these macrophage phenotypes. METHODS: A total of 14 (n) CAD patients were recruited and subsequently grouped into “no apparent CAD”, “non-obstructive CAD” and “obstructive CAD” according to the degree of stenosis. Thirty ml of venous blood was withdrawn to obtain monocyte from the patients. The M1 macrophage was generated by treating the monocyte with GMCSF, LPS and IFN-γ while MCSF, IL-4 and IL-13 were employed to differentiate monocyte into M2 macrophage. After 7 days of polarization, analysis of cell surface differentiation markers (CD86(+)/CD80(+) for M1 and CD206(+)/CD200R(+) for M2) and measurement of MerTK expression were performed using flow cytometry. RESULTS: Both M1 and M2 macrophage expressed similar level of CD86, CD80 and CD206 in all groups of CAD patients. MerTK expression in no apparent CAD patients was significantly higher in M2 macrophage compared to M1 macrophage [12.58 ± 4.40 vs. 6.58 ± 1.37, p = 0.040]. CONCLUSION: Differential polarization of macrophage into M1 and M2 was highly dynamic and can be varied due to the microenvironment stimuli in atherosclerotic plaque. Besides, higher expression of MerTK in patients with the least coronary obstructive suggest its vital involvement in efferocytosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12865-021-00410-2. BioMed Central 2021-03-24 /pmc/articles/PMC7992933/ /pubmed/33761885 http://dx.doi.org/10.1186/s12865-021-00410-2 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Mohd Idrus, Fatin Najiah
Ahmad, Nurul Shuhadah
Hoe, Chee Hock
Azlan, Maryam
Norfuad, Farisha Alia
Yusof, Zurkurnai
Wan Isa, Wan Yus Haniff
Mohamed Ali, Akbar Ali
Yvonne-Tee, Get Bee
Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title_full Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title_fullStr Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title_full_unstemmed Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title_short Differential polarization and the expression of efferocytosis receptor MerTK on M1 and M2 macrophages isolated from coronary artery disease patients
title_sort differential polarization and the expression of efferocytosis receptor mertk on m1 and m2 macrophages isolated from coronary artery disease patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992933/
https://www.ncbi.nlm.nih.gov/pubmed/33761885
http://dx.doi.org/10.1186/s12865-021-00410-2
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