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Maintenance of muscle mass in adult male mice is independent of testosterone

Testosterone is considered a potent anabolic agent in skeletal muscle with a well-established role in adolescent growth and development in males. However, the role of testosterone in the regulation of skeletal muscle mass and function throughout the lifespan has yet to be fully established. While so...

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Autores principales: Davidyan, Arik, Pathak, Suraj, Baar, Keith, Bodine, Sue C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993603/
https://www.ncbi.nlm.nih.gov/pubmed/33764986
http://dx.doi.org/10.1371/journal.pone.0240278
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author Davidyan, Arik
Pathak, Suraj
Baar, Keith
Bodine, Sue C.
author_facet Davidyan, Arik
Pathak, Suraj
Baar, Keith
Bodine, Sue C.
author_sort Davidyan, Arik
collection PubMed
description Testosterone is considered a potent anabolic agent in skeletal muscle with a well-established role in adolescent growth and development in males. However, the role of testosterone in the regulation of skeletal muscle mass and function throughout the lifespan has yet to be fully established. While some studies suggest that testosterone is important for the maintenance of skeletal muscle mass, an understanding of the role this hormone plays in young, adult, and old males with normal and low serum testosterone levels is lacking. We investigated the role testosterone plays in the maintenance of muscle mass by examining the effect of orchiectomy-induced testosterone depletion in C57Bl6 male mice at ages ranging from early postnatal through old age (1.5-, 5-, 12-, and 24-month old mice). Following 28 days of testosterone depletion, we assessed mass and fiber cross-sectional-area (CSA) of the tibialis anterior, gastrocnemius, and quadriceps muscles. In addition, we measured global rates of protein synthesis and degradation using the SuNSET method, western blots, and enzyme activity assays. Twenty-eight days of testosterone depletion resulted in reduced muscle mass in the two youngest cohorts, but had no effect in the two oldest cohorts. Mean CSA decreased only in the youngest cohort and only in the tibialis anterior muscle. Testosterone depletion resulted in a general increase in proteasome activity at all ages. No change in protein synthesis was detected at the terminal time point. These data suggest that within physiological serum concentrations, testosterone may not be critical for the maintenance of muscle mass in mature male mice; however, in young mice testosterone is crucial for normal growth.
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spelling pubmed-79936032021-04-05 Maintenance of muscle mass in adult male mice is independent of testosterone Davidyan, Arik Pathak, Suraj Baar, Keith Bodine, Sue C. PLoS One Research Article Testosterone is considered a potent anabolic agent in skeletal muscle with a well-established role in adolescent growth and development in males. However, the role of testosterone in the regulation of skeletal muscle mass and function throughout the lifespan has yet to be fully established. While some studies suggest that testosterone is important for the maintenance of skeletal muscle mass, an understanding of the role this hormone plays in young, adult, and old males with normal and low serum testosterone levels is lacking. We investigated the role testosterone plays in the maintenance of muscle mass by examining the effect of orchiectomy-induced testosterone depletion in C57Bl6 male mice at ages ranging from early postnatal through old age (1.5-, 5-, 12-, and 24-month old mice). Following 28 days of testosterone depletion, we assessed mass and fiber cross-sectional-area (CSA) of the tibialis anterior, gastrocnemius, and quadriceps muscles. In addition, we measured global rates of protein synthesis and degradation using the SuNSET method, western blots, and enzyme activity assays. Twenty-eight days of testosterone depletion resulted in reduced muscle mass in the two youngest cohorts, but had no effect in the two oldest cohorts. Mean CSA decreased only in the youngest cohort and only in the tibialis anterior muscle. Testosterone depletion resulted in a general increase in proteasome activity at all ages. No change in protein synthesis was detected at the terminal time point. These data suggest that within physiological serum concentrations, testosterone may not be critical for the maintenance of muscle mass in mature male mice; however, in young mice testosterone is crucial for normal growth. Public Library of Science 2021-03-25 /pmc/articles/PMC7993603/ /pubmed/33764986 http://dx.doi.org/10.1371/journal.pone.0240278 Text en © 2021 Davidyan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Davidyan, Arik
Pathak, Suraj
Baar, Keith
Bodine, Sue C.
Maintenance of muscle mass in adult male mice is independent of testosterone
title Maintenance of muscle mass in adult male mice is independent of testosterone
title_full Maintenance of muscle mass in adult male mice is independent of testosterone
title_fullStr Maintenance of muscle mass in adult male mice is independent of testosterone
title_full_unstemmed Maintenance of muscle mass in adult male mice is independent of testosterone
title_short Maintenance of muscle mass in adult male mice is independent of testosterone
title_sort maintenance of muscle mass in adult male mice is independent of testosterone
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993603/
https://www.ncbi.nlm.nih.gov/pubmed/33764986
http://dx.doi.org/10.1371/journal.pone.0240278
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