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LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA

The role of TRPM2-AS lncRNA in OvC has not been explored. This study aimed to investigate whether and how TRPM2-AS contributes to the progression of OvC. First, qRT-PCR was employed to measure the expression of TRPM2-AS, miR-138-5p and SDC3 in OvC samples. A xenograft formation assay was subsequentl...

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Autores principales: Ding, Yi, Tan, Xiangyu, Abasi, Abuduyilimu, Dai, Yun, Wu, Ruxing, Zhang, Tao, Li, Kexin, Yan, Miao, Huang, Xiaoyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993682/
https://www.ncbi.nlm.nih.gov/pubmed/33621194
http://dx.doi.org/10.18632/aging.202541
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author Ding, Yi
Tan, Xiangyu
Abasi, Abuduyilimu
Dai, Yun
Wu, Ruxing
Zhang, Tao
Li, Kexin
Yan, Miao
Huang, Xiaoyuan
author_facet Ding, Yi
Tan, Xiangyu
Abasi, Abuduyilimu
Dai, Yun
Wu, Ruxing
Zhang, Tao
Li, Kexin
Yan, Miao
Huang, Xiaoyuan
author_sort Ding, Yi
collection PubMed
description The role of TRPM2-AS lncRNA in OvC has not been explored. This study aimed to investigate whether and how TRPM2-AS contributes to the progression of OvC. First, qRT-PCR was employed to measure the expression of TRPM2-AS, miR-138-5p and SDC3 in OvC samples. A xenograft formation assay was subsequently performed to detect the tumor growth in vivo. The cell viability, colony formation, cell migration, cell invasion and cell apoptosis were later evaluated using a series of experiments. The western blot assay was utilized to detect the SDC3 protein expression and cell-apoptosis markers. Luciferase reporter gene assay, RIP, and RNA pull-down assays were performed to identify the association between TRPM2-AS, miR-138-5p and SDC3. Findings indicated that the expression of TRPM2-AS and SDC3 was significantly upregulated in OvC tissues and cells, while miR-138-5p expression was significantly downregulated in OvC samples. Unlike miR-138-5p, TRPM2-AS and SDC3 were found to promote OvC development. It was also found that TRPM2-AS could sponge miR-138-5p to release SDC3, thus promoting OvC progression. Apart from that, we discovered that both sh-TRPM2-AS and cisplatin could enhance the apoptosis of OvC cells. Overall, our findings suggested that the TRPM2-AS/miR-138-5p/SDC3 axis was closely associated with OvC tumorigenesis and cisplatin resistance.
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spelling pubmed-79936822021-04-06 LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA Ding, Yi Tan, Xiangyu Abasi, Abuduyilimu Dai, Yun Wu, Ruxing Zhang, Tao Li, Kexin Yan, Miao Huang, Xiaoyuan Aging (Albany NY) Research Paper The role of TRPM2-AS lncRNA in OvC has not been explored. This study aimed to investigate whether and how TRPM2-AS contributes to the progression of OvC. First, qRT-PCR was employed to measure the expression of TRPM2-AS, miR-138-5p and SDC3 in OvC samples. A xenograft formation assay was subsequently performed to detect the tumor growth in vivo. The cell viability, colony formation, cell migration, cell invasion and cell apoptosis were later evaluated using a series of experiments. The western blot assay was utilized to detect the SDC3 protein expression and cell-apoptosis markers. Luciferase reporter gene assay, RIP, and RNA pull-down assays were performed to identify the association between TRPM2-AS, miR-138-5p and SDC3. Findings indicated that the expression of TRPM2-AS and SDC3 was significantly upregulated in OvC tissues and cells, while miR-138-5p expression was significantly downregulated in OvC samples. Unlike miR-138-5p, TRPM2-AS and SDC3 were found to promote OvC development. It was also found that TRPM2-AS could sponge miR-138-5p to release SDC3, thus promoting OvC progression. Apart from that, we discovered that both sh-TRPM2-AS and cisplatin could enhance the apoptosis of OvC cells. Overall, our findings suggested that the TRPM2-AS/miR-138-5p/SDC3 axis was closely associated with OvC tumorigenesis and cisplatin resistance. Impact Journals 2021-02-17 /pmc/articles/PMC7993682/ /pubmed/33621194 http://dx.doi.org/10.18632/aging.202541 Text en Copyright: © 2021 Ding et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Ding, Yi
Tan, Xiangyu
Abasi, Abuduyilimu
Dai, Yun
Wu, Ruxing
Zhang, Tao
Li, Kexin
Yan, Miao
Huang, Xiaoyuan
LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title_full LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title_fullStr LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title_full_unstemmed LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title_short LncRNA TRPM2-AS promotes ovarian cancer progression and cisplatin resistance by sponging miR-138-5p to release SDC3 mRNA
title_sort lncrna trpm2-as promotes ovarian cancer progression and cisplatin resistance by sponging mir-138-5p to release sdc3 mrna
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993682/
https://www.ncbi.nlm.nih.gov/pubmed/33621194
http://dx.doi.org/10.18632/aging.202541
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