Cargando…

Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer

HOXA6 gene plays a role of the oncogene in various cancers. Nonetheless, its effect on gastric cancer (GC) occurrence and development is still unclear. We analysed whether HOXA6 interacts with the PBX2 protein using the STRING database. The molecular mechanism by which HOXA6 synergizes with PBX2 in...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Jianjiao, Zhu, Huiqiong, Hong, Linjie, Tang, Weimei, Wang, Jing, Hu, Hongsong, Wu, Xiaosheng, Chen, Yaying, Liu, Guangnan, Yang, Qiong, Li, Jiaying, Wang, Yusi, Lin, Zhizhao, Xiao, Yizhi, Dai, Weiyu, Huang, Miaojvan, Li, Guoxin, Li, Aimin, Wang, Jide, Xiang, Li, Liu, Side
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993744/
https://www.ncbi.nlm.nih.gov/pubmed/33535170
http://dx.doi.org/10.18632/aging.202426
_version_ 1783669616567910400
author Lin, Jianjiao
Zhu, Huiqiong
Hong, Linjie
Tang, Weimei
Wang, Jing
Hu, Hongsong
Wu, Xiaosheng
Chen, Yaying
Liu, Guangnan
Yang, Qiong
Li, Jiaying
Wang, Yusi
Lin, Zhizhao
Xiao, Yizhi
Dai, Weiyu
Huang, Miaojvan
Li, Guoxin
Li, Aimin
Wang, Jide
Xiang, Li
Liu, Side
author_facet Lin, Jianjiao
Zhu, Huiqiong
Hong, Linjie
Tang, Weimei
Wang, Jing
Hu, Hongsong
Wu, Xiaosheng
Chen, Yaying
Liu, Guangnan
Yang, Qiong
Li, Jiaying
Wang, Yusi
Lin, Zhizhao
Xiao, Yizhi
Dai, Weiyu
Huang, Miaojvan
Li, Guoxin
Li, Aimin
Wang, Jide
Xiang, Li
Liu, Side
author_sort Lin, Jianjiao
collection PubMed
description HOXA6 gene plays a role of the oncogene in various cancers. Nonetheless, its effect on gastric cancer (GC) occurrence and development is still unclear. We analysed whether HOXA6 interacts with the PBX2 protein using the STRING database. The molecular mechanism by which HOXA6 synergizes with PBX2 in GC metastasis is not fully understood. Here, we found that the expression of HOXA6 was increased in GC tissues and cell lines. The upregulation of HOXA6 was closely associated with differentiation, lymph node metastasis, AJCC stage, TNM stage, and poor survival outcome in GC patients based on tissue microarray (TMA) data. Moreover, the overexpression of HOXA6 promoted, whereas siRNA-mediated repression of HOXA6 inhibited, the cell proliferation, migration, and invasion of GC cells. Furthermore, HOXA6 could physically interact with and stabilize PBX2. In addition, HOXA6 and PBX2 expression was positively correlated in GC cells and tissue. HOXA6 and PBX2 suppression in GC cells also led to decreased migration and invasion potential in vitro. In vivo, HOXA6 was shown to cooperate with PBX2 to enhance cell metastasis via orthotopic implantation. These data indicate that HOXA6 promotes cell proliferation, migration, and invasion and that the HOXA6-PBX2 axis may be a useful biomarker for disease progression in GC.
format Online
Article
Text
id pubmed-7993744
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-79937442021-04-06 Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer Lin, Jianjiao Zhu, Huiqiong Hong, Linjie Tang, Weimei Wang, Jing Hu, Hongsong Wu, Xiaosheng Chen, Yaying Liu, Guangnan Yang, Qiong Li, Jiaying Wang, Yusi Lin, Zhizhao Xiao, Yizhi Dai, Weiyu Huang, Miaojvan Li, Guoxin Li, Aimin Wang, Jide Xiang, Li Liu, Side Aging (Albany NY) Research Paper HOXA6 gene plays a role of the oncogene in various cancers. Nonetheless, its effect on gastric cancer (GC) occurrence and development is still unclear. We analysed whether HOXA6 interacts with the PBX2 protein using the STRING database. The molecular mechanism by which HOXA6 synergizes with PBX2 in GC metastasis is not fully understood. Here, we found that the expression of HOXA6 was increased in GC tissues and cell lines. The upregulation of HOXA6 was closely associated with differentiation, lymph node metastasis, AJCC stage, TNM stage, and poor survival outcome in GC patients based on tissue microarray (TMA) data. Moreover, the overexpression of HOXA6 promoted, whereas siRNA-mediated repression of HOXA6 inhibited, the cell proliferation, migration, and invasion of GC cells. Furthermore, HOXA6 could physically interact with and stabilize PBX2. In addition, HOXA6 and PBX2 expression was positively correlated in GC cells and tissue. HOXA6 and PBX2 suppression in GC cells also led to decreased migration and invasion potential in vitro. In vivo, HOXA6 was shown to cooperate with PBX2 to enhance cell metastasis via orthotopic implantation. These data indicate that HOXA6 promotes cell proliferation, migration, and invasion and that the HOXA6-PBX2 axis may be a useful biomarker for disease progression in GC. Impact Journals 2021-02-01 /pmc/articles/PMC7993744/ /pubmed/33535170 http://dx.doi.org/10.18632/aging.202426 Text en Copyright: © 2021 Lin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Lin, Jianjiao
Zhu, Huiqiong
Hong, Linjie
Tang, Weimei
Wang, Jing
Hu, Hongsong
Wu, Xiaosheng
Chen, Yaying
Liu, Guangnan
Yang, Qiong
Li, Jiaying
Wang, Yusi
Lin, Zhizhao
Xiao, Yizhi
Dai, Weiyu
Huang, Miaojvan
Li, Guoxin
Li, Aimin
Wang, Jide
Xiang, Li
Liu, Side
Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title_full Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title_fullStr Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title_full_unstemmed Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title_short Coexpression of HOXA6 and PBX2 promotes metastasis in gastric cancer
title_sort coexpression of hoxa6 and pbx2 promotes metastasis in gastric cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993744/
https://www.ncbi.nlm.nih.gov/pubmed/33535170
http://dx.doi.org/10.18632/aging.202426
work_keys_str_mv AT linjianjiao coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT zhuhuiqiong coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT honglinjie coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT tangweimei coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT wangjing coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT huhongsong coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT wuxiaosheng coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT chenyaying coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT liuguangnan coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT yangqiong coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT lijiaying coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT wangyusi coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT linzhizhao coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT xiaoyizhi coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT daiweiyu coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT huangmiaojvan coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT liguoxin coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT liaimin coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT wangjide coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT xiangli coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer
AT liuside coexpressionofhoxa6andpbx2promotesmetastasisingastriccancer