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Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence

In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields...

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Autores principales: Kesoju, Sandya R., Kramer, Matthew, Brunet, Johanne, Greene, Stephanie L., Jordan, Amelia, Martin, Ruth C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993763/
https://www.ncbi.nlm.nih.gov/pubmed/33765070
http://dx.doi.org/10.1371/journal.pone.0248746
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author Kesoju, Sandya R.
Kramer, Matthew
Brunet, Johanne
Greene, Stephanie L.
Jordan, Amelia
Martin, Ruth C.
author_facet Kesoju, Sandya R.
Kramer, Matthew
Brunet, Johanne
Greene, Stephanie L.
Jordan, Amelia
Martin, Ruth C.
author_sort Kesoju, Sandya R.
collection PubMed
description In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields on adventitious presence (AP) which represents the unwanted presence of a GE gene. Variables other than distance, however, may affect AP. In addition, some AP is often present in the parent seed lots used to establish conventional fields. To identify variables that influence the proportion of AP in conventional alfalfa fields, we performed variable selection regression analyses. Analyses based on a sample-level and a field-level analysis gave similar, though not identical results. For the sample-level model, distance from the GE field explained 66% of the variance in AP, confirming its importance in affecting AP. The area of GE fields within the pollinator foraging range explained an additional 30% of the variation in AP in the model. The density of alfalfa leafcutting bee domiciles influenced AP in both models. To minimize AP in conventional alfalfa seed fields, management practices should focus on optimizing isolation distances while also considering the size of the GE pollen pool within the pollinator foraging range, and the foraging behavior of pollinators.
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spelling pubmed-79937632021-04-05 Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence Kesoju, Sandya R. Kramer, Matthew Brunet, Johanne Greene, Stephanie L. Jordan, Amelia Martin, Ruth C. PLoS One Research Article In insect-pollinated crops, gene flow is affected by numerous factors including crop characteristics, mating system, life history, pollinators, and planting management practices. Previous studies have concentrated on the impact of distance between genetically engineered (GE) and conventional fields on adventitious presence (AP) which represents the unwanted presence of a GE gene. Variables other than distance, however, may affect AP. In addition, some AP is often present in the parent seed lots used to establish conventional fields. To identify variables that influence the proportion of AP in conventional alfalfa fields, we performed variable selection regression analyses. Analyses based on a sample-level and a field-level analysis gave similar, though not identical results. For the sample-level model, distance from the GE field explained 66% of the variance in AP, confirming its importance in affecting AP. The area of GE fields within the pollinator foraging range explained an additional 30% of the variation in AP in the model. The density of alfalfa leafcutting bee domiciles influenced AP in both models. To minimize AP in conventional alfalfa seed fields, management practices should focus on optimizing isolation distances while also considering the size of the GE pollen pool within the pollinator foraging range, and the foraging behavior of pollinators. Public Library of Science 2021-03-25 /pmc/articles/PMC7993763/ /pubmed/33765070 http://dx.doi.org/10.1371/journal.pone.0248746 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Kesoju, Sandya R.
Kramer, Matthew
Brunet, Johanne
Greene, Stephanie L.
Jordan, Amelia
Martin, Ruth C.
Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title_full Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title_fullStr Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title_full_unstemmed Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title_short Gene flow in commercial alfalfa (Medicago sativa subsp. sativa L.) seed production fields: Distance is the primary but not the sole influence on adventitious presence
title_sort gene flow in commercial alfalfa (medicago sativa subsp. sativa l.) seed production fields: distance is the primary but not the sole influence on adventitious presence
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993763/
https://www.ncbi.nlm.nih.gov/pubmed/33765070
http://dx.doi.org/10.1371/journal.pone.0248746
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