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Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants

Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic receptor (Ir)-expressing olfactory sensory neurons in Dros...

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Autores principales: Arguello, J Roman, Abuin, Liliane, Armida, Jan, Mika, Kaan, Chai, Phing Chian, Benton, Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993999/
https://www.ncbi.nlm.nih.gov/pubmed/33666172
http://dx.doi.org/10.7554/eLife.63036
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author Arguello, J Roman
Abuin, Liliane
Armida, Jan
Mika, Kaan
Chai, Phing Chian
Benton, Richard
author_facet Arguello, J Roman
Abuin, Liliane
Armida, Jan
Mika, Kaan
Chai, Phing Chian
Benton, Richard
author_sort Arguello, J Roman
collection PubMed
description Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic receptor (Ir)-expressing olfactory sensory neurons in Drosophila. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not consistently associated with differences in chromatin accessibility, but rather to distinct transcription factor profiles. Genes that are heterogeneously expressed across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration.
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spelling pubmed-79939992021-03-26 Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants Arguello, J Roman Abuin, Liliane Armida, Jan Mika, Kaan Chai, Phing Chian Benton, Richard eLife Developmental Biology Determining the molecular properties of neurons is essential to understand their development, function and evolution. Using Targeted DamID (TaDa), we characterize RNA polymerase II occupancy and chromatin accessibility in selected Ionotropic receptor (Ir)-expressing olfactory sensory neurons in Drosophila. Although individual populations represent a minute fraction of cells, TaDa is sufficiently sensitive and specific to identify the expected receptor genes. Unique Ir expression is not consistently associated with differences in chromatin accessibility, but rather to distinct transcription factor profiles. Genes that are heterogeneously expressed across populations are enriched for neurodevelopmental factors, and we identify functions for the POU-domain protein Pdm3 as a genetic switch of Ir neuron fate, and the atypical cadherin Flamingo in segregation of neurons into discrete glomeruli. Together this study reveals the effectiveness of TaDa in profiling rare neural populations, identifies new roles for a transcription factor and a neuronal guidance molecule, and provides valuable datasets for future exploration. eLife Sciences Publications, Ltd 2021-03-05 /pmc/articles/PMC7993999/ /pubmed/33666172 http://dx.doi.org/10.7554/eLife.63036 Text en © 2021, Arguello et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Arguello, J Roman
Abuin, Liliane
Armida, Jan
Mika, Kaan
Chai, Phing Chian
Benton, Richard
Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title_full Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title_fullStr Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title_full_unstemmed Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title_short Targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
title_sort targeted molecular profiling of rare olfactory sensory neurons identifies fate, wiring, and functional determinants
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993999/
https://www.ncbi.nlm.nih.gov/pubmed/33666172
http://dx.doi.org/10.7554/eLife.63036
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