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Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice

Accumulating evidence suggests that schizophrenia is a disorder of the brain’s communication, a result of functional and structural dysconnectivities. Patients with schizophrenia exhibit irregular neuronal circuit and network activity, but the causes and consequences of such activity remain largely...

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Autores principales: Jones, Zachary B., Zhang, Jiajing, Wu, Yuying, Zhou, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994333/
https://www.ncbi.nlm.nih.gov/pubmed/33776658
http://dx.doi.org/10.3389/fncir.2021.647856
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author Jones, Zachary B.
Zhang, Jiajing
Wu, Yuying
Zhou, Yi
author_facet Jones, Zachary B.
Zhang, Jiajing
Wu, Yuying
Zhou, Yi
author_sort Jones, Zachary B.
collection PubMed
description Accumulating evidence suggests that schizophrenia is a disorder of the brain’s communication, a result of functional and structural dysconnectivities. Patients with schizophrenia exhibit irregular neuronal circuit and network activity, but the causes and consequences of such activity remain largely unknown. Inhibition of 14-3-3 proteins in the mouse brain leads to the expression of multiple schizophrenia endophenotypes. Here we investigated how 14-3-3 inhibition alters neuronal network activity in the mouse hippocampus (HPC) and prefrontal cortex (PFC), key brain regions implicated in schizophrenia pathophysiology. We implanted monopolar recording electrodes in these two regions to record local field potentials both at rest and during a cognitive task. Through our assessment of band power, coherence, and phase-amplitude coupling, we found that neural oscillations in the theta and gamma frequency ranges were altered as a result of 14-3-3 dysfunction. Utilizing transgenic and viral mouse models to assess the effects of chronic and acute 14-3-3 inhibition on oscillatory activities, respectively, we observed several fundamental similarities and differences between the two models. We localized viral mediated 14-3-3 protein inhibition to either the HPC or PFC, allowing us to assess the individual contributions of each region to the observed changes in neural oscillations. These findings identify a novel role of 14-3-3 proteins in neural oscillations that may have implications for our understanding of schizophrenia neurobiology.
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spelling pubmed-79943332021-03-27 Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice Jones, Zachary B. Zhang, Jiajing Wu, Yuying Zhou, Yi Front Neural Circuits Neuroscience Accumulating evidence suggests that schizophrenia is a disorder of the brain’s communication, a result of functional and structural dysconnectivities. Patients with schizophrenia exhibit irregular neuronal circuit and network activity, but the causes and consequences of such activity remain largely unknown. Inhibition of 14-3-3 proteins in the mouse brain leads to the expression of multiple schizophrenia endophenotypes. Here we investigated how 14-3-3 inhibition alters neuronal network activity in the mouse hippocampus (HPC) and prefrontal cortex (PFC), key brain regions implicated in schizophrenia pathophysiology. We implanted monopolar recording electrodes in these two regions to record local field potentials both at rest and during a cognitive task. Through our assessment of band power, coherence, and phase-amplitude coupling, we found that neural oscillations in the theta and gamma frequency ranges were altered as a result of 14-3-3 dysfunction. Utilizing transgenic and viral mouse models to assess the effects of chronic and acute 14-3-3 inhibition on oscillatory activities, respectively, we observed several fundamental similarities and differences between the two models. We localized viral mediated 14-3-3 protein inhibition to either the HPC or PFC, allowing us to assess the individual contributions of each region to the observed changes in neural oscillations. These findings identify a novel role of 14-3-3 proteins in neural oscillations that may have implications for our understanding of schizophrenia neurobiology. Frontiers Media S.A. 2021-03-12 /pmc/articles/PMC7994333/ /pubmed/33776658 http://dx.doi.org/10.3389/fncir.2021.647856 Text en Copyright © 2021 Jones, Zhang, Wu and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Jones, Zachary B.
Zhang, Jiajing
Wu, Yuying
Zhou, Yi
Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title_full Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title_fullStr Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title_full_unstemmed Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title_short Inhibition of 14-3-3 Proteins Alters Neural Oscillations in Mice
title_sort inhibition of 14-3-3 proteins alters neural oscillations in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994333/
https://www.ncbi.nlm.nih.gov/pubmed/33776658
http://dx.doi.org/10.3389/fncir.2021.647856
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