Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging

Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs tha...

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Detalles Bibliográficos
Autores principales: Lu, Xinyue, Zhou, Qiuzhong, Liu, Jin, Sun, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994534/
https://www.ncbi.nlm.nih.gov/pubmed/33786463
http://dx.doi.org/10.1016/j.xpro.2021.100397
Descripción
Sumario:Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs that contribute to aging and to determine their underlying functions in each tissue. We further describe methods to compare the lncRNA expression patterns and dynamic changes among multiple tissues. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).

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