Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging

Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs tha...

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Detalles Bibliográficos
Autores principales: Lu, Xinyue, Zhou, Qiuzhong, Liu, Jin, Sun, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994534/
https://www.ncbi.nlm.nih.gov/pubmed/33786463
http://dx.doi.org/10.1016/j.xpro.2021.100397
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author Lu, Xinyue
Zhou, Qiuzhong
Liu, Jin
Sun, Lei
author_facet Lu, Xinyue
Zhou, Qiuzhong
Liu, Jin
Sun, Lei
author_sort Lu, Xinyue
collection PubMed
description Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs that contribute to aging and to determine their underlying functions in each tissue. We further describe methods to compare the lncRNA expression patterns and dynamic changes among multiple tissues. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020).
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spelling pubmed-79945342021-03-29 Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging Lu, Xinyue Zhou, Qiuzhong Liu, Jin Sun, Lei STAR Protoc Protocol Comprehensive analyses of lncRNAs in aging have been lacking because previous studies have mainly focused on the protein-coding genes during aging. Here, we describe a protocol for the organism-wide analysis of murine lncRNAs during aging. We provide step-by-step instructions to identify lncRNAs that contribute to aging and to determine their underlying functions in each tissue. We further describe methods to compare the lncRNA expression patterns and dynamic changes among multiple tissues. For complete details on the use and execution of this protocol, please refer to Zhou et al. (2020). Elsevier 2021-03-20 /pmc/articles/PMC7994534/ /pubmed/33786463 http://dx.doi.org/10.1016/j.xpro.2021.100397 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Lu, Xinyue
Zhou, Qiuzhong
Liu, Jin
Sun, Lei
Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title_full Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title_fullStr Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title_full_unstemmed Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title_short Protocol for comprehensive RNA sequencing analysis of murine long non-coding RNAs during aging
title_sort protocol for comprehensive rna sequencing analysis of murine long non-coding rnas during aging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994534/
https://www.ncbi.nlm.nih.gov/pubmed/33786463
http://dx.doi.org/10.1016/j.xpro.2021.100397
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AT sunlei protocolforcomprehensivernasequencinganalysisofmurinelongnoncodingrnasduringaging

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