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Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil

Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of t...

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Autores principales: Valladares, Vanessa, Pasquini, Célio, Thiengo, Silvana C., Fernandez, Monica A., Mello-Silva, Clélia C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994760/
https://www.ncbi.nlm.nih.gov/pubmed/33777886
http://dx.doi.org/10.3389/fpubh.2021.636206
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author Valladares, Vanessa
Pasquini, Célio
Thiengo, Silvana C.
Fernandez, Monica A.
Mello-Silva, Clélia C.
author_facet Valladares, Vanessa
Pasquini, Célio
Thiengo, Silvana C.
Fernandez, Monica A.
Mello-Silva, Clélia C.
author_sort Valladares, Vanessa
collection PubMed
description Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of the biomass of living organisms through the interaction between the electromagnetic waves and the sample. NIRS is an innovative, rapid, and non-destructive technique that can contribute to the differentiation of species based on their chemical phenotypes. Chemical profiles were obtained by NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil. The correct identification of these species is important from an epidemiological viewpoint, given that each species has distinct biological and physiological characteristics. The present study aimed to develop a chemometric model for the interspecific and intra-specific classification of the three species, focusing on laboratory and field populations. The data were obtained from 271 live animals, including 150 snails recently collected from the field, with the remainder being raised in the laboratory. Populations were sampled at three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro (B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler® software. The intra-specific classification of the field and laboratory populations obtained accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata). The interspecific differentiation had a hit rate of 75% for the field populations and 80% for the laboratory populations. The results indicate chemical and metabolic differences between populations of the same species from the field and the laboratory. The chemical phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for medical malacology, enabling the systematic discrimination of the Biomphalaria snails that are the intermediate hosts of S. mansoni in Brazil.
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spelling pubmed-79947602021-03-27 Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil Valladares, Vanessa Pasquini, Célio Thiengo, Silvana C. Fernandez, Monica A. Mello-Silva, Clélia C. Front Public Health Public Health Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of the biomass of living organisms through the interaction between the electromagnetic waves and the sample. NIRS is an innovative, rapid, and non-destructive technique that can contribute to the differentiation of species based on their chemical phenotypes. Chemical profiles were obtained by NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil. The correct identification of these species is important from an epidemiological viewpoint, given that each species has distinct biological and physiological characteristics. The present study aimed to develop a chemometric model for the interspecific and intra-specific classification of the three species, focusing on laboratory and field populations. The data were obtained from 271 live animals, including 150 snails recently collected from the field, with the remainder being raised in the laboratory. Populations were sampled at three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro (B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler® software. The intra-specific classification of the field and laboratory populations obtained accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata). The interspecific differentiation had a hit rate of 75% for the field populations and 80% for the laboratory populations. The results indicate chemical and metabolic differences between populations of the same species from the field and the laboratory. The chemical phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for medical malacology, enabling the systematic discrimination of the Biomphalaria snails that are the intermediate hosts of S. mansoni in Brazil. Frontiers Media S.A. 2021-03-12 /pmc/articles/PMC7994760/ /pubmed/33777886 http://dx.doi.org/10.3389/fpubh.2021.636206 Text en Copyright © 2021 Valladares, Pasquini, Thiengo, Fernandez and Mello-Silva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Valladares, Vanessa
Pasquini, Célio
Thiengo, Silvana C.
Fernandez, Monica A.
Mello-Silva, Clélia C.
Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title_full Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title_fullStr Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title_full_unstemmed Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title_short Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
title_sort field application of nir spectroscopy for the discrimination of the biomphalaria species that are intermediate hosts of schistosoma mansoni in brazil
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994760/
https://www.ncbi.nlm.nih.gov/pubmed/33777886
http://dx.doi.org/10.3389/fpubh.2021.636206
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