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Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys
The objective of this study was to evaluate the effects of an hCG subdose applied at the Hou Hai acupoint as an ovulation inducer in donkeys. Eleven donkeys were distributed in randomized blocks in T1 = application of 1,500 IU of hCG intravenous (IV); T2 = 450 IU of hCG applied at the false acupoint...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995259/ https://www.ncbi.nlm.nih.gov/pubmed/33791033 http://dx.doi.org/10.1590/1984-3143-AR2020-0554 |
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author | Ribeiro, Márcio de Oliveira Bittencourt, Rodrigo Freitas Feliciano, Marcus Antônio Rossi Santana, Ana Lúcia Almeida Silva, Mariana Alves de Andrade Felix, Morgana Duarte Santana, Larissa Rodrigues Barbosa, Larissa Pires |
author_facet | Ribeiro, Márcio de Oliveira Bittencourt, Rodrigo Freitas Feliciano, Marcus Antônio Rossi Santana, Ana Lúcia Almeida Silva, Mariana Alves de Andrade Felix, Morgana Duarte Santana, Larissa Rodrigues Barbosa, Larissa Pires |
author_sort | Ribeiro, Márcio de Oliveira |
collection | PubMed |
description | The objective of this study was to evaluate the effects of an hCG subdose applied at the Hou Hai acupoint as an ovulation inducer in donkeys. Eleven donkeys were distributed in randomized blocks in T1 = application of 1,500 IU of hCG intravenous (IV); T2 = 450 IU of hCG applied at the false acupoint (IV), and T3 = 450 IU of hCG applied at the Hou Hai acupoint. There was no difference (P > 0.05) between the treatments regarding the mean diameter of the pre-ovulatory follicle (34.5 ± 1.3 mm), the ovulation rate (96.97%), the interval between induction and ovulation (58.07 ± 16.82 h), the mean diameter of the CL (D0 = 23.0 ± 0.6; D2 = 27.7 ± 1.9 and D8 = 28.2 ± 0.8mm), and serum P(4) concentrations (10.50 ± 2.99 ng.mL(-1)). The application of 450 IU of hCG at the Hou Hai acupoint increased ovulation rate (72.73%) more than 48 h after induction (P = 0.03) and a larger diameter of the CL on D4 (30.7 ± 5.1 mm) (P = 0.04). The vascularization area of the CL on D8, obtained by minimum number of colored pixel (NCP), was greater (P < 0.05) in the donkeys that received 1,500 IU of IV hCG (T1, 41.91 ± 1.17), and we found a positive correlation (P < 0.05) between mean NCP and P(4) concentration in the donkeys that received 450 IU of hCG IV at the false acupoint (T2) or at the Hou Hai acupoint (T3). The application of 450 IU of hCG by IV route at the false acupoint or the Hou Hai acupoint was sufficient to induce ovulation in donkeys, demonstrating that the average dosage commonly used for this species is too high. |
format | Online Article Text |
id | pubmed-7995259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Colégio Brasileiro de Reprodução Animal |
record_format | MEDLINE/PubMed |
spelling | pubmed-79952592021-03-30 Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys Ribeiro, Márcio de Oliveira Bittencourt, Rodrigo Freitas Feliciano, Marcus Antônio Rossi Santana, Ana Lúcia Almeida Silva, Mariana Alves de Andrade Felix, Morgana Duarte Santana, Larissa Rodrigues Barbosa, Larissa Pires Anim Reprod Original Article The objective of this study was to evaluate the effects of an hCG subdose applied at the Hou Hai acupoint as an ovulation inducer in donkeys. Eleven donkeys were distributed in randomized blocks in T1 = application of 1,500 IU of hCG intravenous (IV); T2 = 450 IU of hCG applied at the false acupoint (IV), and T3 = 450 IU of hCG applied at the Hou Hai acupoint. There was no difference (P > 0.05) between the treatments regarding the mean diameter of the pre-ovulatory follicle (34.5 ± 1.3 mm), the ovulation rate (96.97%), the interval between induction and ovulation (58.07 ± 16.82 h), the mean diameter of the CL (D0 = 23.0 ± 0.6; D2 = 27.7 ± 1.9 and D8 = 28.2 ± 0.8mm), and serum P(4) concentrations (10.50 ± 2.99 ng.mL(-1)). The application of 450 IU of hCG at the Hou Hai acupoint increased ovulation rate (72.73%) more than 48 h after induction (P = 0.03) and a larger diameter of the CL on D4 (30.7 ± 5.1 mm) (P = 0.04). The vascularization area of the CL on D8, obtained by minimum number of colored pixel (NCP), was greater (P < 0.05) in the donkeys that received 1,500 IU of IV hCG (T1, 41.91 ± 1.17), and we found a positive correlation (P < 0.05) between mean NCP and P(4) concentration in the donkeys that received 450 IU of hCG IV at the false acupoint (T2) or at the Hou Hai acupoint (T3). The application of 450 IU of hCG by IV route at the false acupoint or the Hou Hai acupoint was sufficient to induce ovulation in donkeys, demonstrating that the average dosage commonly used for this species is too high. Colégio Brasileiro de Reprodução Animal 2020-11-25 /pmc/articles/PMC7995259/ /pubmed/33791033 http://dx.doi.org/10.1590/1984-3143-AR2020-0554 Text en Copyright © The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ribeiro, Márcio de Oliveira Bittencourt, Rodrigo Freitas Feliciano, Marcus Antônio Rossi Santana, Ana Lúcia Almeida Silva, Mariana Alves de Andrade Felix, Morgana Duarte Santana, Larissa Rodrigues Barbosa, Larissa Pires Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title | Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title_full | Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title_fullStr | Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title_full_unstemmed | Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title_short | Subdose of human chorionic gonadotropin applied at the Hou Hai acupoint on follicular dynamics and luteal development in donkeys |
title_sort | subdose of human chorionic gonadotropin applied at the hou hai acupoint on follicular dynamics and luteal development in donkeys |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995259/ https://www.ncbi.nlm.nih.gov/pubmed/33791033 http://dx.doi.org/10.1590/1984-3143-AR2020-0554 |
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