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Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells

Purpose: to examine the lens epithelial cells in diabetic patients with pseudoexfoliation to ultramicroscope and to compare the findings with those of patients without diabetes mellitus (DM) and/or without pseudoexfoliation (PEX). Materials and Methods: Forty patients aged 65-86 years were enrolled...

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Autores principales: Akritidou, Fani, Karachrysaphi, Sofia, Papamitsou, Theodora, Sioga, Antonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Romanian Society of Ophthalmology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995516/
https://www.ncbi.nlm.nih.gov/pubmed/33817432
http://dx.doi.org/10.22336/rjo.2021.8
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author Akritidou, Fani
Karachrysaphi, Sofia
Papamitsou, Theodora
Sioga, Antonia
author_facet Akritidou, Fani
Karachrysaphi, Sofia
Papamitsou, Theodora
Sioga, Antonia
author_sort Akritidou, Fani
collection PubMed
description Purpose: to examine the lens epithelial cells in diabetic patients with pseudoexfoliation to ultramicroscope and to compare the findings with those of patients without diabetes mellitus (DM) and/or without pseudoexfoliation (PEX). Materials and Methods: Forty patients aged 65-86 years were enrolled in the study. All patients had senile cataract and were divided into four groups of ten patients in each group. Group I: patients without pseudoexfoliation, without DM, Group II: without pseudoexfoliation, with DM, Group III: with pseudoexfoliation, without DM, Group IV (Pseudoexfoliation-Diabetic Group): with pseudoexfoliation, with DM. In all cases, part of the central portion of anterior lens capsule was removed during routine cataract surgery, and was properly prepared in order to be examined under a transmission electron microscope. Results: In the control group, mainly degenerative alterations to varying extents were observed. In all groups, intracellular and extracellular oedema, multilayering, apoptosis, completely destroyed cells adjacent to normal cellswere described. In the diabetic group, alterations were more severe with respect to group I. In PEX cases, the additionalirregularity of the epithelium surface, loose intercellular connection, as well as the loose connection between cells and basement membrane were described with the presence of PEX material free and within the basement membrane. In cases with PEX and DM, degenerative alterations and PEX material were observed as well, but the epithelium was better conserved compared to the PEX group. Conclusion: the observed lesions were more extended and more frequent in the pseudoexfoliation group, followed by the diabetic group. The pseudoexfoliation-diabetic group presented less intense modifications raising questions about the interaction of these different diseases. Abbreviations: DM = Diabetes Mellitus, PEX = Pseudoexfoliation, PXM = Pseudoexfoliative Material, AD = Alzheimer disease, TGF-β1 = Transforming Growth Factor beta 1, WHO = World Health Organization, LEC = Lens Epithelium Cells, BM = Basement Membrane, CM = Cytoplasmic Membrane
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spelling pubmed-79955162021-04-01 Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells Akritidou, Fani Karachrysaphi, Sofia Papamitsou, Theodora Sioga, Antonia Rom J Ophthalmol General Articles Purpose: to examine the lens epithelial cells in diabetic patients with pseudoexfoliation to ultramicroscope and to compare the findings with those of patients without diabetes mellitus (DM) and/or without pseudoexfoliation (PEX). Materials and Methods: Forty patients aged 65-86 years were enrolled in the study. All patients had senile cataract and were divided into four groups of ten patients in each group. Group I: patients without pseudoexfoliation, without DM, Group II: without pseudoexfoliation, with DM, Group III: with pseudoexfoliation, without DM, Group IV (Pseudoexfoliation-Diabetic Group): with pseudoexfoliation, with DM. In all cases, part of the central portion of anterior lens capsule was removed during routine cataract surgery, and was properly prepared in order to be examined under a transmission electron microscope. Results: In the control group, mainly degenerative alterations to varying extents were observed. In all groups, intracellular and extracellular oedema, multilayering, apoptosis, completely destroyed cells adjacent to normal cellswere described. In the diabetic group, alterations were more severe with respect to group I. In PEX cases, the additionalirregularity of the epithelium surface, loose intercellular connection, as well as the loose connection between cells and basement membrane were described with the presence of PEX material free and within the basement membrane. In cases with PEX and DM, degenerative alterations and PEX material were observed as well, but the epithelium was better conserved compared to the PEX group. Conclusion: the observed lesions were more extended and more frequent in the pseudoexfoliation group, followed by the diabetic group. The pseudoexfoliation-diabetic group presented less intense modifications raising questions about the interaction of these different diseases. Abbreviations: DM = Diabetes Mellitus, PEX = Pseudoexfoliation, PXM = Pseudoexfoliative Material, AD = Alzheimer disease, TGF-β1 = Transforming Growth Factor beta 1, WHO = World Health Organization, LEC = Lens Epithelium Cells, BM = Basement Membrane, CM = Cytoplasmic Membrane Romanian Society of Ophthalmology 2021 /pmc/articles/PMC7995516/ /pubmed/33817432 http://dx.doi.org/10.22336/rjo.2021.8 Text en ©Romanian Society of Ophthalmology https://creativecommons.org/licenses/by/2.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle General Articles
Akritidou, Fani
Karachrysaphi, Sofia
Papamitsou, Theodora
Sioga, Antonia
Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title_full Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title_fullStr Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title_full_unstemmed Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title_short Pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
title_sort pseudoexfoliation syndrome in diabetic patients: transmission electron microscopy study of anterior lens epithelial cells
topic General Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995516/
https://www.ncbi.nlm.nih.gov/pubmed/33817432
http://dx.doi.org/10.22336/rjo.2021.8
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